sodium-aescinate and Stomach-Neoplasms

To investigate the mechanism of sodium aescinate on the apoptosis of gastric cancer BGC-823 cells and AGS cells.. CCK-8 assay was used to detect the cell viability of cancer cells; cells morphological changes were observed by inverted microscope; changes in nuclear morphology were observed by fluorescence inverted microscope after DAPI staining; cells apoptosis rate was detected by flow cytometry; Western blotting was used to detected the phosphorylation of JAK-1 and STAT-1; laser confocal microscopy was used to observed the nuclear translocation of STAT-1.. Sodium aescinate inhibited cells proliferation in a concentration dependent manner, and changed the cells morphology and nuclear morphology, significantly up-regulated the apoptosis rate of gastric cancer BGC-823 cells and AGS cells, and down-regulated the phosphorylation of JAK-1, STAT-1 protein, inhibited the nuclear translocation of STAT-1.. Sodium aescinate inhibit the proliferation and induce apoptosis in the gastric cancer BGC-823 cells and AGS cells, and this process can be implemented by inhibiting the JAK-1/STAT-1 signaling pathway.

Topics: Apoptosis; Cell Line, Tumor; Cell Proliferation; Cell Survival; Down-Regulation; Humans; Phosphorylation; Saponins; Stomach Neoplasms; Triterpenes; Up-Regulation