sodium-acetate--anhydrous has been researched along with Acquired-Immunodeficiency-Syndrome* in 1 studies
1 other study(ies) available for sodium-acetate--anhydrous and Acquired-Immunodeficiency-Syndrome
| Article | Year |
|---|---|
A validated method for the determination of paracetamol and its glucuronide and sulphate metabolites in the urine of HIV+/AIDS patients using wavelength-switching UV detection.
Paracetamol is a safe drug which has been used as an in-vivo probe to determine phase II metabolism in a HIV+/AIDS population. Due to the biohazard nature of HIV-infected samples, a high performance liquid chromatography (HPLC) assay which offers minimal sample manipulation and maximal specificity was developed. This reverse-phase HPLC method uses wavelength-switching UV detection for the simultaneous determination of paracetamol and its glucuronide and sulfate metabolites in HIV-infected urine samples. The solvent systems involves a simple isocratic elution with a composition of 50 mM sodium acetate buffer, pH adjusted to 3.5; acetonitrile (96:4 v/v) modified with 0.35% trifluroacetic acid. The validated method is highly reproducible with an inter-assay variation of < 7%. This method also shows good precision and sensitivity, making it an ideal assay for phenotyping studies to determine the extent of glucurondiation and sulfation activities. Topics: Acetaminophen; Acetonitriles; Acquired Immunodeficiency Syndrome; Chromatography, High Pressure Liquid; Glucuronates; HIV Seropositivity; Humans; Hydrogen-Ion Concentration; Phenotype; Reproducibility of Results; Sensitivity and Specificity; Sodium Acetate; Sulfates; Trifluoroacetic Acid; Ultraviolet Rays | 1998 |