sinoporphyrin-sodium and Burns

Antibiotic resistance has emerged as one of the most important determinants of outcome in patients with serious infections, along with the virulence of the underlying pathogen. Photodynamic antimicrobial chemotherapy (PACT) has been proposed as an alternative approach for the inactivation of bacteria. This study aims to evaluate the antibacterial effect of sinoporphyrin sodium (DVDMS)-mediated PACT on. Bacteria were incubated with DVDMS and exposed to treatment with light. After PACT treatment, colony-forming units were counted to estimate the bactericidal effect. Intracellular reactive oxygen-species production was detected by flow cytometry. Flow cytometry and fluorescence-microscopy detection of bacterial cell-membrane permeability. Enzyme-linked immunosorbent assays were used to determine expression of VEGF, TGFβ. DVDMS-PACT effectively killed bacterial proliferation. Intracellular ROS levels were enhanced obviously in the PACT-treatment group. SYTO 9 and propidium iodide staining showed a decrease in the ratio of green:red fluorescence intensity in the PACT-treatment group in comparison to the control group. Enzyme-linked immunosorbent-assay results revealed that in the healing process, the expression of bFGF, TGFβ. These results indicate that DVDMS-PACT presents significant bactericidal activity and promotes wound healing after burn infections.

Topics: Animals; Anti-Bacterial Agents; Burns; Drug Resistance, Multiple, Bacterial; Enzyme-Linked Immunosorbent Assay; Female; Light; Mice, Inbred BALB C; Photochemotherapy; Porphyrins; Reactive Oxygen Species; Staphylococcal Infections; Staphylococcus aureus; Transforming Growth Factor beta1; Vascular Endothelial Growth Factor A