sinistrin and Inflammation

Renal elimination of amikacin and other aminoglycosides is slowed down in sepsis-induced acute kidney injury increasing the risk of adverse effects. Since neutrophil gelatinase-associated lipocalin (NGAL) and aminoglycosides share the mechanisms for renal excretion, the predictive power of NGAL was examined towards the changes in amikacin pharmacokinetics during early endotoxemia in anesthetized Wistar rats.. Endogenous biomarkers of inflammation and acute kidney injury were assessed including NGAL in saline-injected controls and two groups of rats challenged with an intravenous injection of bacterial lipopolysaccharide (5 mg/kg)-a fluid-resuscitated group (LPS) and a fluid-resuscitated group infused intravenously with 8 μg/kg/h terlipressin (LPS-T). Sinistrin and amikacin were infused to measure glomerular filtration rate (GFR) and amikacin clearance (CL. Within 3 h of injection, systemic and renal inflammatory responses were induced by lipopolysaccharide. Gene and protein expression of NGAL was increased in the kidneys and the concentrations of NGAL in the plasma (pNGAL) and urine rose 4- to 38-fold (P < 0.01). The decreases in CL. During early endotoxemia in the rat, pNGAL has a moderate predictive ability towards CL

Topics: Acute Kidney Injury; Amikacin; Animals; Biomarkers; Cytokines; Endotoxemia; Glomerular Filtration Rate; Inflammation; Kidney; Lipocalin-2; Lipopolysaccharides; Male; Metabolic Clearance Rate; Models, Animal; Oligosaccharides; Predictive Value of Tests; Rats; Rats, Wistar; Sepsis; Urine