sincalide and Diabetic-Nephropathies

Diabetic nephropathy (DN) is one of the most common microvascular complications of diabetes mellitus. Oxidative stress resulting from high glucose promotes accumulation of ECM and development of DN. Activation of Nrf2 could attenuate oxidative stress and following accumulation of ECM. To find novel therapy for DN, we explored the effects of swinhoeic acid from. CCK-8 and BrdU incorporation assays for survival of mesangial cells gave the concentration of swinhoeic acid in following investigations. ROS, MDA, SOD and CAT were determined. And ECM proteins and their upstream regulators TGF-β. Swinhoeic acid at 10 and 20 μM attenuated oxidative stress and accumulation of ECM in mesangial cells under high glucose. Itactivated Nrf2 in a Keap1-dependent manner, which was involved in its effects.. Swinhoeic acid ameliorates oxidative stress and accumulation of ECM resulting from high glucose in mesangial cells via activating Nrf2 in Keap1-dependent manner.

Topics: Bromodeoxyuridine; Diabetic Nephropathies; Extracellular Matrix; Glucose; Kelch-Like ECH-Associated Protein 1; Mesangial Cells; NF-E2-Related Factor 2; Oxidative Stress; Potentilla; Reactive Oxygen Species; RNA, Small Interfering; Signal Transduction; Sincalide; Superoxide Dismutase