silicon-nitride and Parkinson-Disease

silicon-nitride has been researched along with Parkinson-Disease* in 1 studies

Other Studies

1 other study(ies) available for silicon-nitride and Parkinson-Disease

ArticleYear
Label-free detection of early oligomerization of α-synuclein and its mutants A30P/E46K through solid-state nanopores.
    Nanoscale, 2019, Mar-28, Volume: 11, Issue:13

    A30P and E46K are two mutants of α-synuclein (α-Syn) associated with familial early-onset Parkinson's disease (PD), and amyloid fibrils of α-Syn are the hallmarks of this disease. Detecting the heterogeneous system in the oligomerization stage of α-Syn is crucial for understanding the fibril formation and in vivo toxicity of α-Syn oligomers. Over the last two decades, solid-state nanopore technology has been developed into a reliable and versatile method in single-molecule studies. In this work, we study the time-dependent kinetics of early oligomerization of wild-type α-Syn, A30P, and E46K mutants through silicon nitride solid-state nanopores. By testing A30P, E46K, and wild-type α-Syn samples with different incubation times-from 3 to 15 days-we identify three typical types of oligomers formed in the oligomerization stage and confirm that A30P and E46K mutants aggregate faster than wild-type α-Syn. The results imply that the distinct aggregation pathways and kinetics featured by wild-type α-Syn and mutations may account for their distinct cytotoxicity and pathology in PD-related studies.

    Topics: alpha-Synuclein; Amyloid; Humans; Microscopy, Electron, Transmission; Nanopores; Parkinson Disease; Polymorphism, Single Nucleotide; Silicon Compounds

2019