silicon and Influenza-in-Birds

silicon has been researched along with Influenza-in-Birds* in 2 studies

Other Studies

2 other study(ies) available for silicon and Influenza-in-Birds

Article	Year
Nanowire transistor-based ultrasensitive virus detection with reversible surface functionalization. Chemistry, an Asian journal, 2012, Volume: 7, Issue:9 We have applied a reusable silicon nanowire field-effect transistor (SiNW-FET) as a biosensor to conduct ultrasensitive detection of H5N2 avian influenza virus (AIV) in very dilute solution. The reversible surface functionalization of SiNW-FET was made possible using a disulfide linker. In the surface functionalization, 3-mercaptopropyltrimethoxysilane (MPTMS) was first modified on the SiNW-FET (referred to as MPTMS/SiNW-FET), with subsequent dithiothreitol washing to reduce any possible disulfide bonding between the thiol groups of MPTMS. Subsequently, receptor molecules could be immobilized on the MPTMS/SiNW-FET by the formation of a disulfide bond. The success of the reversible surface functionalization was verified with fluorescence examination and electrical measurements. A surface topograph of the SiNW-FET biosensor modified with a monoclonal antibody against H5N2 virus (referred to as mAb(H5)/SiNW-FET) after detecting approximately 10(-17) M H5N2 AIVs was scanned by atomic force microscopy to demonstrate that the SiNW-FET is capable of detecting very few H5N2 AIV particles. Topics: Animals; Antibodies, Immobilized; Antibodies, Monoclonal; Biosensing Techniques; Birds; Influenza A Virus, H5N2 Subtype; Influenza in Birds; Nanowires; Silanes; Silicon; Surface Properties; Transistors, Electronic	2012
Poly-silicon nanowire field-effect transistor for ultrasensitive and label-free detection of pathogenic avian influenza DNA. Biosensors & bioelectronics, 2009, Jun-15, Volume: 24, Issue:10 Enhanced surveillance of influenza requires rapid, robust, and inexpensive analytical techniques capable of providing a detailed analysis of influenza virus strains. Functionalized poly-crystalline silicon nanowire field-effect transistor (poly-SiNW FET) was demonstrated to achieve specific and ultrasensitive (at fM level) detection of high pathogenic strain virus (H5 and H7) DNA of avian influenza (AI) which is an important infectious disease and has an immediate need for surveillance. The poly-SiNW FET was prepared by a simple and low-cost method that is compatible with current commercial semiconductor process without expensive E-beam lithography tools for large-scale production. Specific electric changes were observed for AI virus DNA sensing when nanowire surface of poly-SiNW FET was modified with complementary captured DNA probe and target DNA (H5) at fM to pM range could be distinguished. With its excellent electric properties and potential for mass commercial production, poly-SiNW FET can be developed to become a portable biosensor for field use and point-of-care diagnoses. Topics: Alphainfluenzavirus; Animals; Base Sequence; Biosensing Techniques; Birds; DNA Probes; DNA, Viral; Electrochemical Techniques; Humans; Influenza in Birds; Nanowires; Population Surveillance; Sensitivity and Specificity; Silicon; Transistors, Electronic	2009

Article

Year

Nanowire transistor-based ultrasensitive virus detection with reversible surface functionalization.

Chemistry, an Asian journal, 2012, Volume: 7, Issue:9

We have applied a reusable silicon nanowire field-effect transistor (SiNW-FET) as a biosensor to conduct ultrasensitive detection of H5N2 avian influenza virus (AIV) in very dilute solution. The reversible surface functionalization of SiNW-FET was made possible using a disulfide linker. In the surface functionalization, 3-mercaptopropyltrimethoxysilane (MPTMS) was first modified on the SiNW-FET (referred to as MPTMS/SiNW-FET), with subsequent dithiothreitol washing to reduce any possible disulfide bonding between the thiol groups of MPTMS. Subsequently, receptor molecules could be immobilized on the MPTMS/SiNW-FET by the formation of a disulfide bond. The success of the reversible surface functionalization was verified with fluorescence examination and electrical measurements. A surface topograph of the SiNW-FET biosensor modified with a monoclonal antibody against H5N2 virus (referred to as mAb(H5)/SiNW-FET) after detecting approximately 10(-17) M H5N2 AIVs was scanned by atomic force microscopy to demonstrate that the SiNW-FET is capable of detecting very few H5N2 AIV particles.

Topics: Animals; Antibodies, Immobilized; Antibodies, Monoclonal; Biosensing Techniques; Birds; Influenza A Virus, H5N2 Subtype; Influenza in Birds; Nanowires; Silanes; Silicon; Surface Properties; Transistors, Electronic

2012

Poly-silicon nanowire field-effect transistor for ultrasensitive and label-free detection of pathogenic avian influenza DNA.

Biosensors & bioelectronics, 2009, Jun-15, Volume: 24, Issue:10

Enhanced surveillance of influenza requires rapid, robust, and inexpensive analytical techniques capable of providing a detailed analysis of influenza virus strains. Functionalized poly-crystalline silicon nanowire field-effect transistor (poly-SiNW FET) was demonstrated to achieve specific and ultrasensitive (at fM level) detection of high pathogenic strain virus (H5 and H7) DNA of avian influenza (AI) which is an important infectious disease and has an immediate need for surveillance. The poly-SiNW FET was prepared by a simple and low-cost method that is compatible with current commercial semiconductor process without expensive E-beam lithography tools for large-scale production. Specific electric changes were observed for AI virus DNA sensing when nanowire surface of poly-SiNW FET was modified with complementary captured DNA probe and target DNA (H5) at fM to pM range could be distinguished. With its excellent electric properties and potential for mass commercial production, poly-SiNW FET can be developed to become a portable biosensor for field use and point-of-care diagnoses.

Topics: Alphainfluenzavirus; Animals; Base Sequence; Biosensing Techniques; Birds; DNA Probes; DNA, Viral; Electrochemical Techniques; Humans; Influenza in Birds; Nanowires; Population Surveillance; Sensitivity and Specificity; Silicon; Transistors, Electronic

2009