shikonin and Subarachnoid-Hemorrhage

To investigate the role of inflammatory response, oxidative damage and changes of ATP-sensitive potassium channels (sK. Time course studies on inflammatory response by real-time PCR, oxidative process and function of isolated basilar artery after SAH in New Zealand White rabbits were performed. Basilar artery smooth muscle cells (BASMCs) in each group were obtained and whole-cell patch-clamp technique was applied to record cell membrane capacitance and K. Inflammatory cytokines levels were highest at 24h compare to 72h after SAH whereas the oxidative damage and cell death marker were at highest peak at 72h. Oxidative damage peak coincided with significant alterations in cell membrane capacitance, K. Currents of ATP-sensitive potassium channels in basilar smooth muscle cells decreased after SAH by putative oxidative modification from immediate inflammatory response and can be protected by shikonin pretreatment.

Topics: Animals; Anti-Inflammatory Agents, Non-Steroidal; Basilar Artery; Cytokines; Female; Inflammation Mediators; KATP Channels; Male; Muscle, Smooth, Vascular; Naphthoquinones; Oxidative Stress; Patch-Clamp Techniques; Rabbits; Subarachnoid Hemorrhage