sepharose and Urinary-Bladder-Neoplasms

The present experimental models of cystic diseases are not adequate and require further investigation.. In this study, a new way of producing a tissue-mimicking model of cysts and cystic neoplasms was evaluated.. To simulate cysts and cystic neoplasms, ex vivo rabbit normal bladders and VX2-implanted tumor bladders were produced, fixed, and embedded in agarose gel.. The samples were classified into four groups based on tumor features and the maximal transverse diameter of the rabbit bladder, which were assessed using computer tomography (CT) imaging and statistically analyzed.. Statistical analysis was performed using Statistical Package for the Social Sciences (SPSS) software. The t-test was used for analyzing enumeration data.. Twenty-one rabbit bladders (21/24) were successfully removed and prepped for this experiment, comprising eleven normal bladders (11/24) and ten implanted with VX2 tumors (10/24). The gelling ingredient used to form the visualization and fixation matrix was agarose at a concentration of 4 g/200 mL. The temperature of the agarose solution was kept constant at 40-45°C, which is the optimal temperature range for ex vivo normal bladder and implanted VX2 tumor bladder insertion. The average time required to embed and fix the bladders in agarose gel was 45.0 ± 5.2 minutes per instance. The gel-fixing matrix's strength and light transmittance were enough for building the models.. We created an experimental tissue-mimicking model of cysts and cystic neoplasms with stable physicochemical features, a safe manufacturing method, and high repeatability. These models may be used to assist with cystic lesion diagnosis and treatment techniques.

Topics: Animals; Cysts; Neoplasms, Cystic, Mucinous, and Serous; Rabbits; Sepharose; Software; Urinary Bladder Neoplasms

Because in vitro cell growth of transitional cell carcinoma explants and cell lines often fail to adequately proliferate in semisolid media, we have examined the effect of agents used to make media semisolid (methyl cellulose, Bacto-agar, Sea Plaque agarose and Sea Prep 15/45 agarose) on the in vitro growth of 11 transitional cell carcinoma cell lines. The growth of human transitional cell carcinoma lines was supported such that agents permissive for growth ranked as follows: Sea Plaque agarose approximately Sea Prep agarose greater than methyl cellulose greater than Bacto-agar. These observations have important implications for the in vitro study of transitional cell carcinoma cell lines and are relevant to the development of improved chemosensitivity determinations for human transitional cell carcinoma.

Topics: Agar; Carcinoma, Transitional Cell; Cell Division; Cell Line; Culture Media; Humans; In Vitro Techniques; Methylcellulose; Sepharose; Urinary Bladder; Urinary Bladder Neoplasms

Topics: Adult; Aged; Cells, Cultured; Culture Media; Cytological Techniques; Female; Humans; Male; Middle Aged; Neoplasm Staging; Neoplastic Stem Cells; Sepharose; Tumor Stem Cell Assay; Urinary Bladder; Urinary Bladder Neoplasms

The effects of different agars (Bacto-agar and deoxycholate lactose agar), agaroses (LE, ME, Sea Plaque and Sea Prep 15/45) and methyl cellulose on the growth of a human tumor cell line, derived from a transitional cell carcinoma of the urinary bladder, were examined. The overall growth in the presence of agars and agarose was generally less than in liquid medium alone. In contrast, growth in the presence of methyl cellulose was significantly enhanced. Thus, methyl cellulose may be a useful agent for optimizing the proliferation of primary tissue cultures prepared from human transitional cell carcinomas.

Topics: Agar; Carcinoma, Transitional Cell; Cell Division; Cell Line; Culture Media; Humans; Methylcellulose; Sepharose; Urinary Bladder Neoplasms