sepharose and Pre-Eclampsia

Exosomes are small (~100 nm) vesicles that carry a wide range of molecules including proteins, RNAs, and DNA. Exosomes are secreted from a wide range of cells including placental cells. Interestingly, exosomes secreted from placental cells have been identified in maternal circulation as early as in 6 weeks of gestation, and their concentration increases with the gestational age. While there is growing interest in elucidating the role of exosomes during normal and complicated pregnancies (such as preeclampsia), progress in the field has been delayed because of the inability to isolate placental exosomes from maternal circulation. Therefore, here we describe a workflow to isolate placental exosomes from maternal circulation.

Topics: Antibodies, Immobilized; Blood Circulation; Blotting, Western; Cell Fractionation; Cross-Linking Reagents; Exosomes; Female; Humans; Immunoconjugates; Immunosorbent Techniques; Placenta; Pre-Eclampsia; Pregnancy; Sepharose; Staphylococcal Protein A