sepharose and Polycythemia-Vera

Anti-erythropoietin antibodies (anti-ESF) were determined by the capacity of the immunoglobulins to neutralize the biological activity of erythropoietin (ESF). Affinity chromatographic methods were used to initiate the purification of anti-ESF by use of ESF covalently linked to Sepharose. It was observed that anti-ESF bound the ESF-Sepharose. In order to remove the nonspecific immunoglobulins, the anti-ESF preparation was subjected to further chromatography on affinity colums where either serum or urinary proteins from a polycythemic patient were covalently linked to Sepharose. It was demonstrated the affinity chromatography could be used as a method isolate anti-ESF.

Topics: Animals; Antibodies; Binding Sites, Antibody; Chromatography, Affinity; Cyanogen Bromide; Epitopes; Erythropoietin; Humans; Immunization; Immunologic Techniques; Neutralization Tests; Polycythemia Vera; Rabbits; Sepharose