sepharose and Periodontitis

The locomotory behaviour of peripheral blood neutrophils (PMNs) from patients with juvenile (JP) and rapidly progressive (RPP) forms of early-onset periodontal disease was studied using the under agarose technique and n-formyl-methionyl-leucyl-phenylalanine (FMLP) as the chemotractant. PMNs from experimental patients showed normal random, chemotactic and chemokinetic locomotory behaviour when compared with control subjects. Further investigation of single-cell movements using time-lapse video analysis also failed to show any significant differences in locomotory behaviour between the PMNs of experimental and control individuals. We conclude that differences in technique may account for much of the variation which exists in the literature with respect to PMN locomotion in periodontal disease. In the final analysis, it is difficult to dispute direct observation of moving cells, and using this approach, we have been unable to confirm the presence of any PMN locomotory defect in our series of patients with early-onset periodontal disease.

Topics: Adolescent; Adult; Aggressive Periodontitis; Cell Movement; Chemotaxis, Leukocyte; Gels; Humans; N-Formylmethionine Leucyl-Phenylalanine; Neutrophils; Periodontal Diseases; Periodontitis; Sepharose; Videotape Recording

Leukocyte migration inhibition by antigen from Candida albicans and Fusobacterium nucleatum was studied in man by means of the direct leukocyte migration agarose technique (LMAT). Antigens were prepared by prolonged ultrasonication and were added to the leukocyte cultures in the original as well as in the concentrated solutions. Significant inhibition of migration with a 10-fold concentrated solution of candida antigen was demonstrated in subjects showing apositive intracutaneous reaction to candida. With the fusobacterial antigen, inhibition of migration or stimulation of migration were demonstrated in subjects with slight peridontal inflammation. A 5-fold increase in the concentration of fusobacterial antigen resulted in increased stimulation of migration or inhibition of migration. Preincubation of the leukocytes with puromycin abolished both inhibition and stimulation of migration by candidal or fusobacterial antigen. The study confirms that it is important to study dose-related effects when applying the LMAT to test cell-mediated immunity. The study is in support of stimulation of migration being an immunologic reaction.

Topics: Antigens, Fungal; Candida albicans; Cell Migration Inhibition; Fusobacterium; Humans; Immunologic Techniques; Periodontitis; Polysaccharides; Puromycin; Sepharose

Topics: Adult; Aged; Antigens, Bacterial; Candida; Cell Migration Inhibition; Culture Media; Dental Plaque; Dentition; Humans; Immunity, Cellular; Leukocyte Count; Leukocytes; Middle Aged; Mouth, Edentulous; Periodontitis; Puromycin; Sepharose; Skin Tests; Tuberculin Test