sepharose and Meningitis--Meningococcal

sepharose has been researched along with Meningitis--Meningococcal* in 1 studies

Other Studies

1 other study(ies) available for sepharose and Meningitis--Meningococcal

Article	Year
A quantitative microassay for leukocyte chemotaxis, using a microscopic slide system with complement-activating yeast particles as gradient source. Journal of immunological methods, 1983, Nov-25, Volume: 64, Issue:3 A simple quantitative microassay was developed for studying polymorphonuclear leukocyte (PMNL) chemotaxis under conditions where the number of available cells is a limiting factor, e.g., pustules, neutropenia, small children and cerebrospinal fluid. PMNL suspensions are placed on glass slides to which fluorescein-labeled yeast particles have been fixed. After adherence, normal human serum is added to the slides. Owing to complement activation, a chemotactic gradient which attracts the adherent PMNL is formed around the yeast particles. The number of PMNL-associated yeast particles in the presence of normal serum is scored, and compared with cells migrating in the presence of inactivated serum or in the absence of serum. A locomotory index is calculated as the number of yeast particles associated with PMNL divided by the total number of yeast particles. Topics: Blood Physiological Phenomena; Chemotaxis, Leukocyte; Child; Complement Activation; Granulocytes; Humans; Immunologic Techniques; Meningitis, Meningococcal; Neutrophils; Saccharomyces cerevisiae; Sepharose	1983

Article

Year

A quantitative microassay for leukocyte chemotaxis, using a microscopic slide system with complement-activating yeast particles as gradient source.

Journal of immunological methods, 1983, Nov-25, Volume: 64, Issue:3

A simple quantitative microassay was developed for studying polymorphonuclear leukocyte (PMNL) chemotaxis under conditions where the number of available cells is a limiting factor, e.g., pustules, neutropenia, small children and cerebrospinal fluid. PMNL suspensions are placed on glass slides to which fluorescein-labeled yeast particles have been fixed. After adherence, normal human serum is added to the slides. Owing to complement activation, a chemotactic gradient which attracts the adherent PMNL is formed around the yeast particles. The number of PMNL-associated yeast particles in the presence of normal serum is scored, and compared with cells migrating in the presence of inactivated serum or in the absence of serum. A locomotory index is calculated as the number of yeast particles associated with PMNL divided by the total number of yeast particles.

Topics: Blood Physiological Phenomena; Chemotaxis, Leukocyte; Child; Complement Activation; Granulocytes; Humans; Immunologic Techniques; Meningitis, Meningococcal; Neutrophils; Saccharomyces cerevisiae; Sepharose

1983