sepharose and Liver-Cirrhosis

Properties of soft biological tissues are increasingly used in medical diagnosis to detect various abnormalities, for example, in liver fibrosis or breast tumors. It is well known that mechanical stiffness of human organs can be obtained from organ responses to shear stress waves through Magnetic Resonance Elastography. The Local Interaction Simulation Approach is proposed for effective modelling of shear wave propagation in soft tissues. The results are validated using experimental data from Magnetic Resonance Elastography. These results show the potential of the method for shear wave propagation modelling in soft tissues. The major advantage of the proposed approach is a significant reduction of computational effort.

Topics: Algorithms; Breast Neoplasms; Computer Simulation; Elasticity Imaging Techniques; Finite Element Analysis; Gelatin; Humans; Liver; Liver Cirrhosis; Models, Statistical; Phantoms, Imaging; Sepharose; Shear Strength; Software; Stress, Mechanical

A modified radioimmunoassay for conjugated cholates is presented. In this modification the antibody is chemically bound to Sepharose which enablproducibility (the coefficient of variation between samples is 4%). The whole procedure is carried out at room temperature and with a short incubation time (45 min). Serum can by analysed directly (no extractions or modifications needed). The assay is a suitable tool for liver function testing. A rough indication of total bile acid concentration in serum or bile can also be obtained with this assay.

Topics: Cholic Acids; Cross Reactions; Hepatitis; Humans; Kinetics; Liver Cirrhosis; Radioimmunoassay; Sepharose; Serum Albumin, Bovine