sepharose and Hyperprolactinemia

sepharose has been researched along with Hyperprolactinemia* in 2 studies

Other Studies

2 other study(ies) available for sepharose and Hyperprolactinemia

ArticleYear
Macroprolactin detection by precipitation with protein A-sepharose: a rapid screening method compared with polyethylene glycol precipitation.
    Clinical chemistry, 2003, Volume: 49, Issue:3

    Topics: Adolescent; Adult; Aged; Chemical Precipitation; Child; Female; Humans; Hyperprolactinemia; Immunoassay; Male; Middle Aged; Polyethylene Glycols; Prolactin; Sepharose; Staphylococcal Protein A

2003
Rapid detection of macroprolactin in the form of prolactin-immunoglobulin G complexes by immunoprecipitation with anti-human IgG-agarose.
    Clinical chemistry and laboratory medicine, 2001, Volume: 39, Issue:12

    Immunoprecipitation with anti-human immunoglobulin G-agarose was evaluated for the detection of prolactin-immunoglobulin G (PRL-IgG) complexes in macroprolactinemic samples from hyperprolactinemic patients (prolactin measured using the automated Elecsys PRL assay; Roche Diagnostics, Mannheim, Germany). Using the polyethylene glycol (PEG) precipitation test on serum samples with PRL above 1000 mIU/l, we detected macroprolactin in 38 out of 175 samples with a recovery below 50%. Gel filtration chromatography on a subset of hyperprolactinemic samples confirmed that macroprolactin was the predominant immunoreactive form of PRL in samples which showed a recovery < or = 50%. In 37 out of 38 samples containing macroprolactin, immunoprecipitation with anti-human IgG (anti-hIgG)-agarose revealed a higher PRL-binding (19.1-91.3%) than in the sera containing monomeric PRL (< 10%). In conclusion, we found that PEG precipitation detected macroprolactin in 21.7% of samples with elevated PRL levels as measured by the Elecsys PRL assay, and that in the vast majority of these samples the presence of PRL-IgG complexes could be demonstrated by immunoprecipitation with anti-hIgG-agarose. In view of the high prevalence of PRL-IgG complexes in hyperprolactinemic patients and the simplicity of the test, immunoprecipitation with anti-hIgG-agarose might play a role in the routine laboratory handling of hyperprolactinemic samples, especially with regards to PRL assays where PEG causes interference.

    Topics: Antigen-Antibody Complex; Humans; Hyperprolactinemia; Immunoglobulin G; Polyethylene Glycols; Precipitin Tests; Prolactin; Reproducibility of Results; Sepharose

2001