sepharose and Hyperlipidemias

Topics: Aspirin; Blood Glucose; Chemistry, Clinical; Chromatography, Affinity; Chromatography, High Pressure Liquid; Chromatography, Ion Exchange; Electrophoresis, Agar Gel; Glycated Hemoglobin; Glycosylation; Hemoglobins; Humans; Hyperlipidemias; Immunoassay; Jaundice; Nephelometry and Turbidimetry; Quality Control; Renal Insufficiency; Sepharose; Specimen Handling; Terminology as Topic

The hypolipidemic and antioxidant effects of porphyran from the red algae Porphyra haitanensis as a dietary supplement were evaluated in mice. The levels of serum TC, TG and LDL-C in MP group increased significantly (p<0.05 or p<0.01) by 28.5%, 29.4% and 33.5% compared with the model group. These significant rises were accompanied by significant declines of plasma HDL-C by 21.6% compared with the model group. In addition, the liver content of malondialdehyde significantly decreased, while the superoxide dismutase, catalase, glutathione peroxidase activities significantly increased. The levels of serum SOD and GSH-Px in MP group increased significantly by 51.2% and 99.6% compared with the model group. The results suggested that porphyran could be used as functional foods and natural drugs in preventing the hyperlipidemia and this activity might be attributed to its antioxidant potential.

Topics: Animals; Antioxidants; Catalase; Glutathione Peroxidase; Hyperlipidemias; Hypolipidemic Agents; Male; Malondialdehyde; Mice; Porphyra; Sepharose; Superoxide Dismutase

A simple, low-priced chromatographic system to generate plasma lipoprotein profiles from total human plasma was tested with plasma from normolipidemic subjects and patients with heterozygous familial hypercholesterolemia, hyper-alpha-lipoproteinemia, lipoprotein lipase deficiency, familial dysbetalipoproteinemia and familial lecithin-cholesterol-acyl-transferase deficiency. The system appears to be a good alternative to more expensive high-pressure liquid chromatography systems, notably in lipoprotein laboratories already provided with equipment for column chromatography. If a microplate photometer and a computer is available in the laboratory, the measurement of various lipids in 70-80 eluant fractions from the columns can be simplified.

Topics: Adult; Cholesterol; Chromatography, Liquid; Female; Humans; Hyperlipidemias; Lipoproteins; Lipoproteins, HDL; Lipoproteins, LDL; Lipoproteins, VLDL; Male; Middle Aged; Reproducibility of Results; Sepharose; Triglycerides

Polymorphism at the apolipoprotein E (ApoE) locus is an important factor in the development of remnant (Type III) hyperlipidemia and also influences the distribution of cholesterol concentrations in the population. The new method for ApoE phenotyping described here gives good results with simple apparatus. Serum (10 microL) is digested with sialidase (EC 3.2.1.18), delipidated, and redissolved in 6 mol/L urea. Electrofocusing is carried out in agarose, followed by immunoblotting with a monoclonal antibody to ApoE and an anti-immunoglobulin-peroxidase conjugate. Sialidase-catalyzed digestion effectively removes sialated forms of ApoE, which eases interpretation. This method can be used in nonspecialist laboratories and is particularly suited for assay of large numbers of samples.

Topics: Antibodies, Monoclonal; Apolipoproteins E; Cysteamine; Humans; Hydrolysis; Hyperlipidemias; Immunoblotting; Isoelectric Focusing; Neuraminidase; Phenotype; Polymorphism, Genetic; Sepharose

The lipid-lowering effect of pantethine, a new drug affecting lipid metabolism, had been evaluated in carbohydrate-induced hyperlipidemic rats. Administration of the drug raised post-heparin lipolytic activities, the change being due to an increase in lipoprotein lipase activity, whereas hepatic lipase activity remained virtually unchanged. Total lipoprotein lipase activity per g of adipose tissue increased in pantethine-treated rats compared with controls. Furthermore, the soluble lipoprotein lipase of fat-pads was fractionated by heparin-Sepharose affinity chromatography. The first active peak, originated from the microsomal fractions, significantly increased after the drug treatment, while the second one, originated from the plasma membranes, remained unchanged. The increase in the microsomal lipoprotein lipase activity may be due to an increase in intracellular synthesis of lipoprotein lipase enzyme proteins. The heterogeneity of lipoprotein lipase of rat adipose tissues was ensured using affinity chromatography on heparin-Sepharose.

Topics: Adipose Tissue; Animals; Body Weight; Chromatography, Agarose; Epididymis; Hyperlipidemias; Lipids; Lipoprotein Lipase; Liver; Male; Organ Size; Pantetheine; Rats; Rats, Inbred Strains; Sepharose; Subcellular Fractions; Sulfhydryl Compounds

A modified heparin-Sepharose affinity chromatography procedure (Boberg et al., J. Lipid Res. 18:544-547, 1977) was developed to determine two different triglyceride lipase activities in human post-heparin plasma: hepatic triglyceride lipase (I) and lipoprotein lipase (II). With this procedure, lipoproteins were separated from the eluted lipases. The total lipolytic activity of II was eluted from heparin-Sepharose by heparin. The use of heparin as eluting agent prevents the partial inhibition of II, in contrast to the procedure based on elution of II with a high concentration of NaCl. In a comparative study with the modified heparin-Sepharose affinity column chromatography, the immunochemical and protamine sulfate inhibition procedures, the results indicated that these three procedures are equally suitable for the determination of I and II from normolipidemic subjects. However, because of possible interference by plasma, the column-chromatographic procedure is the preferred method for measuring lipase concentrations in post-heparin plasma of hyperlipidemic patients. The II activity of post-heparin plasma from normolipidemic subjects was not significantly age-(20-39 and 40-60 years) or sex-related. I activity was also not significantly different with respect to age, but was significantly greater in men than in women.

Topics: Adult; Age Factors; Female; Heparin; Humans; Hyperlipidemias; Kinetics; Lipase; Lipid Mobilization; Lipoprotein Lipase; Male; Middle Aged; Reference Values; Sepharose; Sex Factors

Topics: Age Factors; Child, Preschool; Cholesterol; Electrophoresis, Agar Gel; Humans; Hyperlipidemias; Lipoproteins; Lipoproteins, HDL; Lipoproteins, VLDL; Microchemistry; Sepharose

Reduction of plasma-cholesterol by the removal of low-density lipoproteins (L.D.L.) in an extracorporeal system is described as a possible approach in the treatment of familial hypercholesterolaemia. L.D.L. were removed from the blood by their interaction with heparin linked to agarose beads in the presence of calcium ions. Plasma-L.D.L. was markedly decreased in two patients with heterozygous familial hypercholesterolaemia. The technique is specific for the removal of L.D.L., as the concentration of high-density lipoproteins was not affected. The treatment was well tolerated by all three subjects (i.e., two hypercholesterolaemic patients and a normal volunteer), and there were no undesirable effects. Several haematological parameters, clinical-chemistry tests, including serum enzymes, and immunoelectrophoresis of plasma proteins were all unaffected by the treatment.

Topics: Calcium; Cholesterol; Chromatography, Affinity; Extracorporeal Circulation; Follow-Up Studies; Heparin; Heterozygote; Humans; Hypercholesterolemia; Hyperlipidemias; Lipoproteins, HDL; Lipoproteins, LDL; Male; Middle Aged; Plasmapheresis; Sepharose

Topics: Blood Protein Electrophoresis; Humans; Hyperlipidemias; Lipoproteins; Sepharose

1. A low cost method for lipoprotein electrophoresis in agarose gel is described. The basic procedure, described by Noble, has been modified for use with a Durrum cell which is commonly used for paper electrophoreis. 2. Up to 28 samples can be analyzed per Durrum cell in 4 hours. 3. A staining technique, which involves filtration of a suspension of Sudan Black B, allows for rapid staining and provides satisfactory reproducibility from run to run. 4. In a small number of patients a double pre-beta band is seen, and is different from sinking pre-beta and floating beta lipoproteins.

Topics: Electrophoresis; Humans; Hyperlipidemias; Lipoproteins; Lipoproteins, HDL; Lipoproteins, LDL; Lipoproteins, VLDL; Sepharose; Staining and Labeling