sepharose and Hematologic-Diseases

The polyamines associated to human erythrocytes from healthy donors are mainly localized intracellularly. In fact chromatography of the erythrocytes on a resin which has a high affinity and capacity for polyamines does not affect the amount of polyamines associated to the erythrocytes. The low ability of spermine to adsorb to the external surface of erythrocytes at physiological ionic strength is suggested also by studies conducted with sealed ghosts. Also erythrocytes from patients with hematological and dermatological diseases which contain increased levels of polyamines show an intracellular location of these amines.

Topics: Chromatography, Affinity; Erythrocyte Membrane; Erythrocytes; Hematologic Diseases; Humans; Polyamines; Sepharose; Skin Diseases