sepharose and Gram-Negative-Bacterial-Infections

The aim of study was to determine the influence of zinc chelate, valnemulin and it's combination on Brachyspira hyodysenteriae shedding and morphological changes of colonic mucosa in an experimental model of swine dysentery (SD). The study was performed on pigs coming from a dysentery-free herd. Animals were inoculated by B. hyodysenteriae strain B204. When the clinical signs of SD and B. hyodysenteriae shedding developed, the pigs were divided into four treatment groups. The first group was treated with zinc chelate (250 ml/1000 L in water), second group was given valnemulin in feed at 75 ppm; the third group was given a combination of both and the fourth group was control. The results demonstrated therapeutic effect of valnemulin in pigs with serious SD and did not show therapeutic effect of chelated zinc.

Topics: Animals; Brachyspira hyodysenteriae; Colon; Diterpenes; Drug Therapy, Combination; Dysentery; Gram-Negative Bacterial Infections; Immunohistochemistry; Intestinal Mucosa; Sepharose; Swine; Swine Diseases

The structural component of Gram- bacteria, endotoxin (ET), induces the release of endogenous mediators of sepsis. Attempts to remove these downstream molecules in vivo, have not improved survival. However, extracorporeal strategies such as continuous renal replacement therapy or therapeutic plasmapheresis have shown benefit. We are presenting an affinity-based extracorporeal technology for the removal of ET from whole blood. The small-scale device contains an adsorbent that removed 75% of ET present in whole blood. This affinity resin displayed good hemocompatibility regarding the coagulation pathway. Minimal platelet, neutrophil and complement activation were observed. There was also no evidence of consumption of coagulation factors or cell loss. In as much as ET participates in both the inflammatory and coagulation abnormalities in sepsis, this method represents an efficient and hemocompatible way to remove ET from whole blood, which, in an extracorporeal setting, may improve the outcome of sepsis.

Topics: Adsorption; Anti-Infective Agents; Blood Coagulation Factors; Cell Count; Chromatography, Affinity; Complement C3a; Endotoxins; Fibrinogen; Gram-Negative Bacterial Infections; Hemostasis; Humans; Leukocyte Elastase; Ligands; Monocytes; Ofloxacin; Sepharose; Shock, Septic; Transforming Growth Factor beta; Tumor Necrosis Factor-alpha