sepharose and Fascioliasis

A glycoprotein (27 kDa) was isolated from crude somatic antigen of Fasciola gigantica by two steps affinity chromatography and was used in early detection of experimental fasciolosis in cattle by indirect ELISA and in dot-ELISA formats. Although, anti-27 kDa antibodies could be detected after 3 weeks post infection (WPI) by dot - ELISA which was one week later than indirect ELISA. The test, dot-ELISA, was more convenient in field application. By the test (dot-ELISA) the infection could be equally detected in animals infected with 100, 200 and 300 metacercariae of F. gigantica with high sensitivity. Further, the antigen (27 kDa) was not found to react with goat sera infected with Paramphistomum epiclitum, which are giving strong reaction to homologous immature and mature fluke antigens of P. epiclitum.

Topics: Animals; Antibodies, Helminth; Antigens; Antigens, Helminth; Cattle; Cyanogen Bromide; Electrophoresis, Polyacrylamide Gel; Enzyme-Linked Immunosorbent Assay; Fasciola; Fascioliasis; Glycoproteins; Goats; Immunoglobulin G; Lymnaea; Rabbits; Sensitivity and Specificity; Sepharose; Time Factors; Trematode Infections

Topics: Age Factors; Animals; Antigens; Cattle; Fasciola hepatica; Fascioliasis; Fluorescent Antibody Technique; Freeze Drying; Humans; Immunoglobulin G; Rabbits; Sepharose

Topics: Alanine; Aminobutyrates; Aminohippuric Acids; Antigens; Citrulline; Cross Reactions; Cyanogen Bromide; Ethanolamines; Fasciola hepatica; Fascioliasis; Fluorescent Antibody Technique; Glutamates; Glycine; Humans; Phosphoric Acids; Schistosoma mansoni; Schistosomiasis; Sepharose; Serum Albumin, Bovine; Taurine