sepharose and Cat-Diseases

Eleven dogs with spontaneous neoplasms were intensively treated with an immunoadsorption system consisting of a continuous flow centrifuge, Protein A-Sepharose columns, and a semi-automatic elution system. Despite consistent and substantial lowering of immunoglobulin G levels, tumor regression was noted in only one of 11 dogs. In contrast, infusion of small volumes of plasma after incubation with heat and formalin-treated Staphylococcus aureus Cowan I resulted in a tumoricidal response in five of six animals. These results suggest that tumor necrosis is probably not induced by Protein A-mediated removal of humoral "blocking" factors.

Topics: Animals; Cat Diseases; Cats; Dog Diseases; Dogs; Female; Immunoglobulin G; Male; Neoplasms; Perfusion; Plasma; Sepharose; Staphylococcal Protein A; Staphylococcus aureus