sepharose and Carcinoma--Lewis-Lung

sepharose has been researched along with Carcinoma--Lewis-Lung* in 1 studies

Other Studies

1 other study(ies) available for sepharose and Carcinoma--Lewis-Lung

Article	Year
Isolation of a 41-kDa protein with cell adhesion activity for animal tumor cells from the mushroom Hypsizigus marmoreus by affinity chromatography with type IV collagen immobilized on agarose. Bioscience, biotechnology, and biochemistry, 2000, Volume: 64, Issue:4 A type IV collagen-binding protein of 41 kDa was isolated from the mushroom Hypsizigus marmoreus and the protein was designated as HM41. The Western blotting analysis with anti-HM41 antibodies demonstrated that HM41 was unrelated to HM23, which had been shown to have an affinity for type IV collagen. The microsequence analysis of the membrane-blotted peptides generated by fragmentation with cyanogen bromide showed no homologous proteins reported. HM41 had cell adhesion-promoting activity for murine Lewis lung carcinoma LL2 cells and human fibrosarcoma HT1080 cells. These results indicate that HM41 is a hitherto undescribed fungus protein that can interact both with animal extracellular matrix protein type IV collagen and with animal tumor cells. Topics: Agaricales; Animals; Carcinoma, Lewis Lung; Cell Adhesion; Chromatography, Affinity; Collagen; Fibrosarcoma; Fungal Proteins; Humans; Integrins; Mice; Receptors, Collagen; Sepharose; Tumor Cells, Cultured	2000

Article

Year

Isolation of a 41-kDa protein with cell adhesion activity for animal tumor cells from the mushroom Hypsizigus marmoreus by affinity chromatography with type IV collagen immobilized on agarose.

Bioscience, biotechnology, and biochemistry, 2000, Volume: 64, Issue:4

A type IV collagen-binding protein of 41 kDa was isolated from the mushroom Hypsizigus marmoreus and the protein was designated as HM41. The Western blotting analysis with anti-HM41 antibodies demonstrated that HM41 was unrelated to HM23, which had been shown to have an affinity for type IV collagen. The microsequence analysis of the membrane-blotted peptides generated by fragmentation with cyanogen bromide showed no homologous proteins reported. HM41 had cell adhesion-promoting activity for murine Lewis lung carcinoma LL2 cells and human fibrosarcoma HT1080 cells. These results indicate that HM41 is a hitherto undescribed fungus protein that can interact both with animal extracellular matrix protein type IV collagen and with animal tumor cells.

Topics: Agaricales; Animals; Carcinoma, Lewis Lung; Cell Adhesion; Chromatography, Affinity; Collagen; Fibrosarcoma; Fungal Proteins; Humans; Integrins; Mice; Receptors, Collagen; Sepharose; Tumor Cells, Cultured

2000