sepharose and Bile-Duct-Diseases

In recent years, with the widespread use of laparoscopic cholecystectomy and liver transplantation, complications involving the biliary system are increasing. All techniques available have a high risk of recurrence or high-morbidity problems. We developed a three-dimensional collagen duct modified with agarose hydrogel, to substitute the affected extrahepatic bile duct.. We used it in 40 guinea pigs and studied the histology and physiology at 4 wk, 3, and 6 mo after transplantation. Blood test, histologic techniques, and cholangiography were performed in all of them.. All experimental animals survived up to their sacrifices. Our graft showed highly potential applications to treat hepatobiliary diseases that require surgery.

Topics: Animals; Bile Duct Diseases; Bile Ducts, Extrahepatic; Biocompatible Materials; Cattle; Collagen; Gels; Guinea Pigs; Liver Diseases; Models, Animal; Sepharose; Tissue Engineering