sepharose and Arteriosclerosis

Topics: Animals; Arteriosclerosis; Blood Component Removal; Dextran Sulfate; Extracorporeal Circulation; Heparin; Humans; Hyperlipoproteinemia Type II; Lipoproteins, LDL; Plasmapheresis; Sepharose; Sorption Detoxification

Human serum was found to contain enzyme activities hydrolyzing succinyl trialanine paranitroanilide and 3H-kappa-elastin Sepharose substrates. Both types of activities could be partly abolished by serine active site titrants (phenylmethanesulfonylfluoride, diisopropylphosphorofluoridate) and partly by neutral chelating agents (EDTA; 1-10-phenanthroline). The combination of phenylmethanesulfonylfluoride and EDTA gave a complete inhibition of human serum elastase-type activities indicating the presence of at least two different types of elastases (serine and metalloproteases) in human serum. In nonsmokers, the average serum elastase-type activity on succinyl trialanine paranitroanilide was found equal to 78.1 ng/ml porcine pancreatic elastase equivalents and on 3H-kappa-elastin sepharose beads equal to 688.8 ng/ml. No statistically significant differences were observed in elastase levels in the sera of individuals presenting clinical symptoms of atherosclerosis. The sera of patients suffering from chronic obstructive lung diseases contained, however, higher amounts of elastase-type activities, respectively equal to 237.2 ng/ml on succinyl trialanine paranitroanilide and 1,096 ng/ml on 3H-kappa-elastin Sepharose beads and was quantitatively significant when compared with control subjects.

Topics: Adult; Aged; Arteriosclerosis; Elastin; Humans; Lung Diseases, Obstructive; Middle Aged; Oligopeptides; Pancreatic Elastase; Sepharose; Substrate Specificity