securinine and Lung-Neoplasms

Lung cancer remains the leading cause of cancer-related death worldwide. It is important to explore the biomarkers of diagnosis and prognosis in lung cancer. To evaluate the cytotoxicity of L-securinine and the expression and methylation of secreted frizzled-related proteins (SFRPs) genes in the human lung adenocarcinoma cells, cell counting kit-8 (CCK-8) assay was used to assess the proliferation of lung adenocarcinoma cells treated with L-securinine. Quantitative real-time PCR (qRT-PCR) and bisulfite sequencing PCR were used to detect the expression and the DNA methylation of SFRPs genes, respectively. L-securinine inhibited the proliferation of lung adenocarcinoma cells and induced the upregulation of SFRP1 gene expression and the methylation changes at CpG sites in the SFRP1 promoter region. L-securinine was a potential agent in the treatment of lung cancer by upregulation of SFRP1 gene expression and changing the SFRP1 gene methylation.

Topics: Adenocarcinoma; Adenocarcinoma of Lung; Azepines; Cell Line, Tumor; DNA Methylation; Heterocyclic Compounds, Bridged-Ring; Humans; Intracellular Signaling Peptides and Proteins; Lactones; Lung Neoplasms; Molecular Structure; Piperidines; Promoter Regions, Genetic; Proteins; Stereoisomerism