sch-442416 and Glaucoma

Müller cells are principal glial cells in rat retina and have attracted much attention in glaucoma studies. However, it is not clear whether adenosine and adenosine receptor (AR) antagonists play any roles in the regulation of potassium channels in Müller cells and subsequently in the promotion of glutamine synthetase (GS) and L-Glutamate/L-Aspartate Transporter (GLAST) functions. We found that chronic ocular hypertension (COH) in rat down-regulated Müller cells Kir2.1, Kir4.1, TASK-1, GS and GLAST expressions and attenuated the peak of inward potassium current. Retinal ganglion cells (RGC) count was lower in the COH rats than that in the sham operation animals. Intravitreal injection of selective A2A AR antagonist SCH442416 up-regulated Müller cell Kir4.1, TASK-1, GS and GLAST expressions and enhanced inward potassium currents compared with those in the COH rats with vehicle control. Meanwhile, the RGC count was higher following intravitreal injection of SCH442416 in the COH rats than that after vehicle injection. The fact that PKA inhibitor H-89 blocked these SCH442416 effects suggested that the PKA signaling pathway was involved in the observed ocular responses following the intravitreal SCH442416 injection.

Topics: Adenosine; Amino Acid Transport System X-AG; Animals; Cyclic AMP-Dependent Protein Kinases; Ependymoglial Cells; Glaucoma; Glutamate-Ammonia Ligase; Isoquinolines; Male; Nerve Tissue Proteins; Patch-Clamp Techniques; Potassium Channels; Potassium Channels, Inwardly Rectifying; Potassium Channels, Tandem Pore Domain; Purinergic P1 Receptor Antagonists; Pyrazoles; Pyrimidines; Rats; Rats, Sprague-Dawley; Sulfonamides