saxitoxin and Carcinoma--Squamous-Cell

A BALB/c murine monoclonal antibody against the trichothecene mycotoxin T-2 was generated. The antibody, designated HD11, specifically bound T-2 mycotoxin. The binding of HD11 to T-2 conjugated to bovine serum albumin was inhibited by free T-2 toxin but not by the water-soluble heterocyclic guanidines saxitoxin and tetrodotoxin. The T-2 detection limit in an enzyme-linked immunosorbent assay with HD11 was in the nanogram range. The in vitro cytotoxicity of T-2, as measured by the inhibition of radiolabeled leucine uptake of the human epidermoid carcinoma Hep-2 and KB cell lines, was completely reversed by the addition of HD11. Rabbit anti-idiotypic antibodies specific for HD11 were generated and characterized.

Topics: Antibodies, Anti-Idiotypic; Antibodies, Monoclonal; Carcinoma, Squamous Cell; Cell Survival; Enzyme-Linked Immunosorbent Assay; Humans; Immunoglobulin Idiotypes; Saxitoxin; Serum Albumin, Bovine; Sesquiterpenes; T-2 Toxin; Tetrodotoxin; Tumor Cells, Cultured