saquinavir and Necrosis

saquinavir has been researched along with Necrosis* in 1 studies

Other Studies

1 other study(ies) available for saquinavir and Necrosis

ArticleYear
Vascular endothelial toxicity induced by HIV protease inhibitor: evidence of oxidant-related dysfunction and apoptosis.
    Cardiovascular toxicology, 2004, Volume: 4, Issue:2

    HIV-protease inhibitor (HIV-PI) drugs are critical for highly active antiretroviral therapy (HAART) efficacy, but several recent reports have suggested that metabolic and/or cardiovascular toxicities are associated with these drugs. Given the importance of the HIV-PI drug class and the widespread and chronic use of these agents in an expanding patient population, further understanding of this potential drug toxicity is imperative. Here, we investigated a role for direct endothelial toxicity induced by saquinavir (SAQ), the first HIV-PI drug marketed in the United States and still an important component of HAART therapies. In initial studies using isolated vascular tissues, we observed selective impairment of endothelium-dependent vasodilation with no effect on contractile responses. Subsequent studies using human endothelial cells in culture at clinically relevant concentrations (5 and 10 microM, 2-48 h) demonstrated concentration-dependent increases in cell death, mainly via apoptosis rather than necrosis (determined via Annexin-V positive membrane labeling). Live cell imaging also demonstrated increased intracellular oxidant production (as measured by DCF fluorescence), which could be abrogated by incubation with the antioxidant N-acetylcysteine (NAC). NAC also prevented SAQ- induced apoptotic cell death. These data demonstrate that SAQ has direct toxicological effects on endothelial cells, and that the toxicity apparently involves apoptotic pathway activation via reactive oxygen and/or nitrogen species.

    Topics: Animals; Aorta, Thoracic; Apoptosis; Cell Line; Cell Survival; Endothelial Cells; Endothelium, Vascular; HIV Protease Inhibitors; Humans; In Vitro Techniques; Male; Necrosis; Rats; Rats, Sprague-Dawley; Reactive Oxygen Species; Saquinavir; Umbilical Veins; Vasodilation

2004