sapogenins and Carbon-Tetrachloride-Poisoning

The effects of saikosaponin-d extracted from the roots of Buplerum falcatum L. on increased toxicity of CCl4 and increased activities of microsomal enzymes induced by phenobarbitone have been examined. Saikosaponin-d showed protection against the CCl4-hepatotoxicity enhanced by phenobarbitone. It also inhibited increases in the content of cytochrome P450 and NADPH-cytochrome c reductase activity, which are induced by the phenobarbitone treatment, but the spectral characteristics of P450 were not altered. The rate of microsomal lipid peroxidation by NADPH and CCl4 was significantly lowered in-vitro in rats pretreated with phenobarbitone and saikosaponin-d compared with those pretreated with phenobarbitone alone.

Topics: Alanine Transaminase; Animals; Anti-Inflammatory Agents; Carbon Tetrachloride Poisoning; Drug Synergism; Enzyme Induction; Lipid Peroxides; Liver; Male; Microsomes, Liver; NADPH-Ferrihemoprotein Reductase; Oleanolic Acid; Phenobarbital; Rats; Rats, Inbred Strains; Sapogenins; Saponins; Time Factors

Topics: Animals; Carbon Tetrachloride Poisoning; Liver; Liver Cirrhosis, Experimental; Oleanolic Acid; Rats; Sapogenins

Topics: Animals; Carbon Tetrachloride Poisoning; Female; Liver; Male; Oleanolic Acid; Rats; Sapogenins; Triglycerides

The effects of saikosaponin-d extracted from the roots of Bupleurum falcatum L. on carbon tetrachloride-induced hepatic injury were studied in rats. Pretreatment with saikosaponin-d produced a remarkable inhibitory action on acute hepatic injury by CCl4. A significant inhibition of lipid peroxidation induced by an acute dose of CCl4 in the liver of rats pre-treated with saikosaponin-d was also noted. Continuous injection of CCl4 caused liver cirrhosis in rats but the severity of cirrhosis was reduced in rats treated simultaneously with CCl4 and saikosaponin-d.

Topics: Animals; Body Weight; Carbon Tetrachloride Poisoning; Chemical and Drug Induced Liver Injury; Lipid Peroxides; Male; Microsomes, Liver; Necrosis; Oleanolic Acid; Organ Size; Plants, Medicinal; Rats; Rats, Inbred Strains; Sapogenins; Saponins