sanglifehrin-a and Melanoma

sanglifehrin-a has been researched along with Melanoma* in 1 studies

Other Studies

1 other study(ies) available for sanglifehrin-a and Melanoma

ArticleYear
Curcumin-induced melanoma cell death is associated with mitochondrial permeability transition pore (mPTP) opening.
    Biochemical and biophysical research communications, 2014, May-23, Volume: 448, Issue:1

    Here we studied the role of mitochondrial permeability transition pore (mPTP) opening in curcumin's cytotoxicity in melanoma cells. In cultured WM-115 melanoma cells, curcumin induced mitochondrial membrane potential (MPP) decrease, cyclophilin-D (CyPD)-adenine nucleotide translocator 1 (ANT-1) (two mPTP components) mitochondrial association and cytochrome C release, indicating mPTP opening. The mPTP blocker sanglifehrin A (SfA) and ANT-1 siRNA-depletion dramatically inhibited curcumin-induced cytochrome C release and WM-115 cell death. CyPD is required for curcumin-induced melanoma cell death. The CyPD inhibitor cyclosporin A (CsA) or CyPD siRNA-depletion inhibited curcumin-induced WM-115 cell death and apoptosis, while WM-115 cells with CyPD over-expression were hyper-sensitive to curcumin. Finally, we found that C6 ceramide enhanced curcumin-induced cytotoxicity probably through facilitating mPTP opening, while CsA and SfA as well as CyPD and ANT-1 siRNAs alleviated C6 ceramide's effect on curcumin in WM-115 cells. Together, these results suggest that curcumin-induced melanoma cell death is associated with mPTP opening.

    Topics: Adenine Nucleotide Translocator 1; Cell Death; Cell Line, Tumor; Curcumin; Cyclophilins; Cytochromes c; Humans; Lactones; Melanoma; Mitochondrial Membrane Transport Proteins; Mitochondrial Permeability Transition Pore; Peptidyl-Prolyl Isomerase F; Spiro Compounds

2014