salvianolic-acid-a and Memory-Disorders

To investigate the protective effects of Salvianolic acid A against impairment of memory induced by cerebral ischemia-reperfusion in mice and the relationship between antioxidant activity of salvianolic acid A and its protective effects on brain injury caused by reperfusion.. Step down and step through tests were used to examine the function of memory in the model of impairment of memory induced by cerebral ischemia-reperfusion in mice.. Salvianolic acid A (Sal A) at the dosage of 3 and 10 mg.kg-1 i.v. could improve the impaired memory function induced by cerebral ischemia-reperfusion as shown in step down and step through tests. In step down tests, the mean number of errors of Sal A 3 and 10 mg.kg-1 treated group were 1.29, 1.15 and that of control group was 3.8 (P < 0.01). The latencies of these tests were longer than those of the control group. Meanwhile, 3 and 10 mg.kg of Sal A i.v. were found to reduce the malondialdehyde contents in the cortex, hippocampus and corpus striatum of cerebral ischemia-reperfusion rat in vivo. Sal A 10-100 nmol.L-1 was also shown to inhibit lipid-peroxidation of the brain and scavenge the free hydroxyl radicals in vitro.. These indicate that the ameliorating effect of Sal A on learning and memory impairment caused by cerebral ischemia reperfusion may be related to its anti-oxidant activity.

Topics: Animals; Antioxidants; Avoidance Learning; Brain Ischemia; Caffeic Acids; Drugs, Chinese Herbal; Lactates; Lipid Peroxidation; Male; Memory Disorders; Mice; Plant Extracts; Rats; Rats, Wistar; Reperfusion Injury; Salvia miltiorrhiza

In the present experiments, an impairment of memory model was made by cerebral ischemia-reperefusion in mice. Sal A at the dosage of 3 and 10 mg.kg-1 i.v. was shown to improve the impairment of memory function induced by cerebral ischemia-reperefusion in step down and step through tests. In these tests, the number of errors of Sal A treated group was less and the latency was longer than that of control group. Meanwhile, Sal A 3 and 10 mg.kg-1 i.v. was found to reduce the MDA contents in the cortex, hippocampus and striatum of cerebral ischemia-reperfused rats in vivo. Sal A 10-100 nmol.L-1 was shown to inhibit the brain lipid-peroxidation and scavenge the free hydroxyl radical in vitro. These results indicate that the antagonistic effects of Sal A on impairment of learning and memory caused by cerebral ischemia-reperefusion may be related with its anti-oxidant activity.

Topics: Animals; Brain; Brain Ischemia; Caffeic Acids; Drugs, Chinese Herbal; Free Radical Scavengers; Lactates; Male; Memory Disorders; Mice; Rats; Reperfusion Injury