salvianolic-acid-a and Hyperlipidemias

In this study, untargeted lipidomics based on UPLC-Q/TOF-MS, network pharmacology and atomic force microscopy were used to explore the common biomarkers of hyperlipidemia and coronary heart disease, the therapeutic mechanism of the main components of Salvia miltiorrhiza as well as the action mechanism of key lipids. Firstly, the serum samples of 30 healthy people, 30 patients with coronary heart disease and 30 patients with hyperlipidemia were analyzed by using lipidomics technology to obtain biomarkers which can be used to link hyperlipidemia and coronary heart disease and to find potential targets; then, the key components and core targets of Salvia miltiorrhiza intervention in hyperlipidemia and coronary heart disease were analyzed by network pharmacology, the results were verified by atomic force microscopy. It showed that SMS2 might be the key target. And through network pharmacology and atomic force microscope analysis, it can be inferred that salvianolic acid A can combine with SMS2 to play a therapeutic role.

Topics: Caffeic Acids; Chromatography, High Pressure Liquid; Coronary Disease; Female; Healthy Volunteers; Humans; Hyperlipidemias; Lactates; Lipidomics; Male; Membrane Proteins; Metabolic Networks and Pathways; Microscopy, Atomic Force; Middle Aged; Nerve Tissue Proteins; Network Pharmacology; Salvia miltiorrhiza; Transferases (Other Substituted Phosphate Groups)

To investigate the synergistic effect of salvianolic acids (Sals) and tanshinones (Tans), to compare the pharmacodynamic effect of Danshen co-microemulsion, salvianolic acids microemulsion, tanshinones microemulsion, tanshinones suspension and blank microemulsion on hemorheology in rats with hyperlipidemia.. Fat milk was administered to SD rats in the morning for 23 days, and drugs were given in the afternoon since the 13th day. On the 14th day, the blood viscosity, haematocrit and platelet aggregation were determined.. Both Tans and Sals could inhibit the platelet aggregation, and decrease the blood viscosity. The effect of Sals on the decrease of blood viscosity was much more significant than that of other groups. And Tans could significantly inhibit the platelet aggregation than others. Compared to Tans or Sals used separately, co-administration of Tans and Sals at the same time could decrease the blood viscosity and inhibit the platelet aggregation significantly.. As a new drug carrier, the microemulsion system can increase tanshinone solubility dramatically and improve its bioavailability. And co-mecroemulsion of Tans and Sals can produce the synergy of Tans and Sals preferably and are much more efficient than either Tans microemulsion or Sals microemulsion used alone.

Topics: Abietanes; Animals; Blood Viscosity; Caffeic Acids; Cholesterol; Drug Carriers; Drug Synergism; Drugs, Chinese Herbal; Emulsions; Hyperlipidemias; Lactates; Male; Phenanthrenes; Platelet Aggregation; Random Allocation; Rats; Rats, Sprague-Dawley; Salvia miltiorrhiza; Triglycerides