salvianolic-acid-B and Hypertension

Salvianolic acid B (Sal B) is one of the most abundant phenolic acids derived from the root of Danshen with potent anti-oxidative properties. The present study examined the vasoprotective effect of Sal B in hypertensive mice induced by angiotensin II (Ang II). Sal B (25mg/kg/day) was administered via oral gavage for 11days to Ang II (1.2mg/kg/day)-infused C57BL/6J mice (8-10weeks old). The vascular reactivity (both endothelium-dependent relaxations and contractions) in mouse arteries was examined by wire myography. The production of reactive oxygen species (ROS), protein level and localization of angiotensin AT

Topics: Angiotensin II; Animals; Benzofurans; Dose-Response Relationship, Drug; Drugs, Chinese Herbal; Endothelium, Vascular; Human Umbilical Vein Endothelial Cells; Humans; Hypertension; Male; Mice; Mice, Inbred C57BL; Organ Culture Techniques; Treatment Outcome

The roots of Salviae miltiorrhiza (RSM) have been traditionally used for treatment of hypertensive disease in China, Korea, and Japan. Bioassay guided fractionation and purification as assayed by angiotensin converting enzyme (ACE) inhibitory assay resulted in the isolation of lithospermic acid B (LSB) as an active principle. The ACE plasma activities were significantly inhibited by the addition of LSB in a dose-dependent manner of which IC50 value was 86 microg/ml (120 microM). Moreover, angiotensin I-induced contraction was markedly attenuated by prior exposure of endothelium-intact aortic rings to LSB. These results suggest that RSM-induced antihypertensive effect may be, at least in part, due to ACE inhibitory effect of LSB.

Topics: Angiotensin-Converting Enzyme Inhibitors; Animals; Aorta; Benzofurans; Depsides; Dose-Response Relationship, Drug; Humans; Hypertension; Inhibitory Concentration 50; Male; Muscle Contraction; Phytotherapy; Plant Extracts; Plant Roots; Rats; Rats, Sprague-Dawley; Salvia miltiorrhiza