salubrinal and Osteoarthritis

To examine the role of mechanical force and hypoxia on chondrocytes apoptosis and osteoarthritis (OA)-liked pathological change on mandibular cartilage through over-activation of endoplasmic reticulum stress (ERS).. We used two in vitro models to examine the effect of mechanical force and hypoxia on chondrocytes apoptosis separately. The mandibular condylar chondrocytes were obtained from three-week-old male Sprague-Dawley rats. Flexcell 5000T apparatus was used to produce mechanical forces (12%, 0.5Hz, 24h vs 20%, 0.5Hz, 24h) on chondrocytes. For hypoxia experiment, the concentration of O. Tunicamycin, 20% mechanical forces and hypoxia (1% O. We confirmed that mechanical stress and local hypoxia both contributed to the chondrocytes apoptosis. Mechanical stress can cause OA-like pathological change in rat mandibular condylar cartilage via ERS activation and hypoxia existed in the meantime. Both mechanical forces and hypoxia can induce ERS and cause chondrocytes apoptosis only if the stimulate was in higher level. Salubrinal can protect chondrocytes from apoptosis, and relieve OA-liked pathological change on mandibular condylar cartilage under mechanical stress stimulation.

Topics: Animals; Apoptosis; Blotting, Western; Cartilage, Articular; Chondrocytes; Cinnamates; Endoplasmic Reticulum Stress; Hypoxia; Immunohistochemistry; In Situ Nick-End Labeling; In Vitro Techniques; Male; Mandibular Condyle; Osteoarthritis; Rats; Rats, Sprague-Dawley; Real-Time Polymerase Chain Reaction; Stress, Mechanical; Thiourea; Tunicamycin