salpichrolide-g and Carcinoma--Hepatocellular

Thirty-seven naturally occurring withanolides (1-37), previously isolated in our laboratories, were evaluated for their potential to induce quinone reductase with cultured murine hepatoma cells (Hepa 1c1c7). Spiranoid (29, 32) and 18-functionalized withanolides (2-5, 7-9, 24) were found to be potent inducers of the enzyme, while 5alpha-substituted derivatives exhibited weak activity. Preliminary studies were performed with compound 29 to evaluate enzyme-inducing capacity in multiple organ sites of BALB/c mice. Significant induction was observed in liver and colon, but not in lung, stomach, or mammary gland.

Topics: Animals; Brassicaceae; Breast; Carcinoma, Hepatocellular; Colon; Enzyme Induction; Inhibitory Concentration 50; Liver; Lung; Mice; Mice, Inbred BALB C; Molecular Structure; Nuclear Magnetic Resonance, Biomolecular; Plants, Medicinal; Quinone Reductases; Solanaceae; Stereoisomerism; Steroids; Stilbenes; Stomach; Tumor Cells, Cultured