salicylates and Root-Resorption

This study evaluated the response of apical and periapical tissues of dogs' teeth with pulp vitality after root canal filling with the endodontic sealers Sealapex Xpress and Real Seal XT.. Thirty-eight root canals with vital pulp from dogs' premolars were used. After instrumentation, the canals were filled with Sealapex Xpress and gutta-percha (group SX/GP, n = 16) or Real Seal XT and Resilon cones (group RS/R, n = 22). The animals were killed after 90 days, and the teeth with surrounding tissues were subjected to histotechnical processing. Hematoxylin-eosin-stained sections were examined by conventional light microscopy for a quantitative histopathologic analysis (sealing of apical opening by newly formed mineralized tissue [biological sealing], inflammatory cell infiltrate, root and bone tissue resorption), according to a scoring system. The subsequent sections were evaluated by immunohistochemistry for identification of mineralization markers (osteopontin, alkaline phosphatase, and RUNX2). Data were analyzed by nonparametric Mann-Whitney U test (α = 0.05).. Complete biological sealing was observed in 50% and 22.7% of the specimens of groups SX/GP and RS/R, respectively. Partial biological sealing was observed in 25% and 54.6% and absence of sealing in 25% and 22.7% of the specimens of groups SX/GP and RS/R, respectively. There were no significant differences (P > .05) between the groups for the scores attributed to the histopathologic parameters. Positive staining for osteopontin, alkaline phosphatase, and RUNX2 was observed in both groups, especially in the periodontal ligament.. Sealapex Xpress and RealSeal XT feature tissue compatibility in vivo and allow for sealing of apical opening by deposition of mineralized tissue.

Topics: Alkaline Phosphatase; Alveolar Bone Loss; Animals; Apexification; Biocompatible Materials; Calcium Hydroxide; Composite Resins; Core Binding Factor Alpha 1 Subunit; Dental Bonding; Dogs; Gutta-Percha; Inflammation; Osteopontin; Periapical Tissue; Periodontal Ligament; Root Canal Filling Materials; Root Canal Obturation; Root Canal Preparation; Root Resorption; Salicylates

The major concern in the therapeutics of tooth replantation refers to the occurrence of root resorption and different approaches have been proposed to prevent or treat these complications. The purpose of this study was to evaluate tissue response to delayed replantation of anterior rat teeth treated endodontically using calcium hydroxide, Sealapex, and Endofill without the placement of gutta-percha cones. Thirty rats had their right upper incisor extracted and maintained in dry storage for 60 min. After removal of the dental papilla, enamel organ, pulp tissue, and periodontal ligament remnants, the teeth were immersed in 2% sodium fluoride phosphate acidulated, pH 5.5, for 10 min. The root canals were dried with absorbent paper points and the teeth were assigned to three groups (n = 10) according to the filling material. Group I - calcium hydroxide and propyleneglycol paste, Group II - Sealapex, and Group III - Endofill. The sockets were irrigated with saline and the teeth were replanted. Replacement resorption, inflammatory resorption and ankylosis were observed in all groups. Although the occurrence of inflammatory resorption was less frequent in Group I, there were no statistically significant differences among the groups. It may be concluded that compared to the paste, filling the root canals with Sealapex and Endofill sealers without the placement of gutta-percha cones did not provide better results.

Topics: Acidulated Phosphate Fluoride; Alveolar Process; Animals; Calcium Hydroxide; Connective Tissue; Dental Cementum; Dental Pulp Cavity; Dentin; Fibroblasts; Incisor; Lymphocytes; Macrophages; Male; Periodontal Ligament; Rats; Rats, Wistar; Retrograde Obturation; Root Canal Filling Materials; Root Canal Irrigants; Root Resorption; Salicylates; Tooth Ankylosis; Tooth Replantation; Tooth Socket; Wound Healing

This paper describes a case of autogenous tooth transplantation. An external inflammatory root resorption developed in a 30-year-old female patient and was successfully treated with the placement of calcium hydroxide-based intracanal dressings. Autotransplantation of tooth 32 into the extraction socket of tooth 30 was carried out with no intraoperative complications and uneventful postoperative course. However, the patient did not appear on the 14th postoperative day to initiate endodontic therapy of the transplanted tooth. She did not return for 3 months, at which time the clinical and periapical radiographic examinations revealed the presence of external inflammatory root resorption. Endodontic treatment was initiated immediately. Root canals were prepared and filled with a calcium hydroxide-based intracanal dressing (Calen paste), changed every 21 days during a 6-month period, and were thereafter obturated with Sealapex calcium hydroxide-based sealer and gutta-percha points. After 2 years of follow-up, the transplanted tooth was symptom free with no exacerbated sensibility to percussion, normal mobility, occlusion, and masticatory function. Probing revealed no periodontal pockets or other pathological signs. Radiographic examination showed the periradicular area with normal appearance, completely healed resorption areas, and presence of an intact lamina dura.

Topics: Adult; Calcium Hydroxide; Female; Gutta-Percha; Humans; Molar, Third; Oral Surgical Procedures; Root Canal Filling Materials; Root Canal Irrigants; Root Canal Therapy; Root Resorption; Salicylates; Tooth Socket; Transplantation, Autologous

The objective of this study was to evaluate the apical and periapical repair after endodontic treatment of teeth with pulp necrosis and a chronic periapical lesion in dogs. Seventy-two root canals from four mongrel dogs were submitted to biomechanical preparation, using 5.25% sodium hypochlorite or 2% chlorhexidine digluconate as the irrigating solution. The root canals were subsequently either filled immediately with Sealapex, using active lateral gutta-percha condensation, or a calcium hydroxide root canal dressing was applied for 15 days before filling with Sealapex. After 210 days, the animals were killed by anesthetic overdose, and the obtained histological sections were stained with hematoxylin and eosin for optical microscopic analysis of apical and periapical repair. There was better histological repair in the groups with the root canal dressing (p < 0.05) than the groups with immediate obturation. Comparing the immediate obturation groups, irrigation with chlorhexidine solution resulted in better repair than sodium hypochlorite.

Topics: Animals; Bone Resorption; Calcium Hydroxide; Chlorhexidine; Dental Pulp Necrosis; Disease Models, Animal; Disinfectants; Dogs; Female; Gutta-Percha; Male; Periapical Diseases; Periapical Periodontitis; Periapical Tissue; Periodontal Ligament; Root Canal Filling Materials; Root Canal Irrigants; Root Canal Obturation; Root Canal Preparation; Root Canal Therapy; Root Resorption; Salicylates; Sodium Hypochlorite; Statistics as Topic; Statistics, Nonparametric; Time Factors; Wound Healing

To test an electrical device for determining root canal length in primary teeth in vitro, and to compare it with the radiographic length measurement.. Two examiners determined the root canal length of 24 extracted maxillary primary incisors (12 with visible root resorption and 12 without) using an electrical root canal meter (Tri Auto ZX, Morita, Dietzenbach, Germany). The instrument was left in the root canal after the second examination and a radiograph was taken. The whole tooth was cleared by immersion in methylsalicylate and subsequently photographed. Both radiographs and photographs of cleared teeth were measured and compared with the electronic measurements.. The evaluation of the radiographs showed a mean distance between the instrument tips and apices of 0.60 (+/- 0.41) mm; evaluation of the cleared teeth showed an equivalent distance of 0.62 (+/- 0.40) mm. The mean distance between instrument tip and acceptable working length (determined with the clearing method) was 0.26 (+/- 0.24) mm in teeth without resorption and 0.29 (+/- 0.30) mm in teeth with resorption.. The presence of resorption in primary teeth did not affect the accuracy of electrical measurement of root canal length in vitro. The application of this method in primary teeth should be evaluated further.

Topics: Confidence Intervals; Dental Pulp Cavity; Electric Conductivity; Electronics, Medical; Equipment Design; Fixatives; Humans; Image Processing, Computer-Assisted; Incisor; Observer Variation; Odontometry; Photography; Radiography; Reproducibility of Results; Root Resorption; Salicylates; Statistics as Topic; Surface Properties; Tooth Apex; Tooth, Deciduous

The purpose of this study was to investigate long-term pH changes in cavities prepared in root surface dentin of extracted teeth after obturation of the root canal with gutta-percha and a variety of sealers containing calcium hydroxide. After cleaning and shaping, root canals in 50 recently extracted, human single-rooted teeth were divided into five groups. Each of four groups was obturated with gutta-percha and either Sealapex, Sealer 26, Apexit, or CRCS, all of which contain calcium hydroxide. The remaining group served as the control and was not obturated with gutta-percha or sealer. Cavities were prepared in the facial surface of the roots in the cervical and middle regions. The pH was measured in these dentinal cavities at the initiation of the experiment, and 3, 7, 14, 21, 28, 45, 60, 90, and 120 days after obturation. Results indicate that the pH at the surface of the root does not become alkaline when calcium hydroxide cements are used as root canal sealers. Regardless of the sealer used, the observed pattern of pH change was not different from that seen in the control group of roots that were not treated with sealer. It is concluded that calcium hydroxide-containing cements, although suitable for use as root canal sealants, do not produce an alkaline pH at the root surface. If such a pH change is related to treatment of root resorption, these sealants do not contribute to this treatment.

Topics: Bismuth; Calcium Hydroxide; Dentin; Dentin Permeability; Diffusion; Humans; Hydrogen-Ion Concentration; Hydroxyl Radical; Root Canal Filling Materials; Root Resorption; Salicylates; Surface Properties; Tooth Cervix; Tooth Root; Zinc Oxide

Topics: Calcium Hydroxide; Humans; Root Resorption; Salicylates