salicylates and Peritonitis

Topics: Adrenal Cortex Hormones; Arthritis; Electrocardiography; Humans; Inflammation; Morphine; Myocardial Infarction; Myositis; Pericarditis; Peritonitis; Pleurisy; Pneumonia; Salicylates

Resveratrol is a natural compound with a plethora of activities as well as limitations. We recently reported a series of resveratrol-salicylate analogs with potential chemopreventive activity. Herein, we report the anti-inflammatory and antioxidant properties of these resveratrol derivatives. Using an in vitro COX inhibition assay, and two in vivo protocols (carrageenan-induced peritonitis and paw edema), we identified a novel compound (C10) as a potent anti-inflammatory agent. The enhanced potency of C10 was associated with the ability of C10 to decrease the activity of myeloperoxidase (MPO) enzyme at 10mg/kg, whereas resveratrol and it's natural analog (TMS) did not exert the same effect. Additionally, C10 significantly reduced the concentration of intracellular reactive oxygen species. Because of the proven association between cancer, inflammation, and oxidative stress, we believe that C10 is a promising chemopreventive molecule.

Topics: Administration, Oral; Animals; Anti-Inflammatory Agents, Non-Steroidal; Antioxidants; Carrageenan; Cyclooxygenase Inhibitors; Dose-Response Relationship, Drug; Edema; Hep G2 Cells; Humans; Hydrogen Peroxide; Mice; Molecular Structure; Oxidative Stress; Peritonitis; Prostaglandin-Endoperoxide Synthases; Reactive Oxygen Species; Resveratrol; Salicylates; Stilbenes; Structure-Activity Relationship

Thermal imaging has been utilised, both preclinically and clinically, as a tool for assessing inflammation. Psoriasis is a chronic inflammatory skin disease characterised by hyperkeratosis, dermal inflammatory infiltrate and increased angiogenesis. The aim of the present study was to assess the usefulness of thermography in psoriatic lesion regression after topical treatment with bee propolis, recognised as potent antioxidants and anti-inflammatory agents.. We monitored the inflammation process induced by irritants such as n-Hexyl salycilate (HXS) or Di-n-Propyl Disulfide (PPD) by histopatological assessment of the skin, thermographic scanning, total number of inflammatory cells in the peritoneal cavity, differential analysis of cells in the peritoneal cavity, macrophage spreading index, haematological and biochemical parameters, frequencies of micronucleated reticulocytes, lipid peroxidation and glutathione assay in the skin.. Topically applied ethanolic extract of propolis (EEP) with HXS or PPD reduced the lipid peroxidation in the skin and total number of inflammatory cells in the skin and peritoneal cavity, functional activity of macrophages, the number of micronuclei in mouse peripheral blood reticulocytes and enzymatic activity of ALP and AST.. These results demonstrate that topical application of EEP may improve psoriatic-like skin lesions by suppressing functional activity of macrophages and ROS production. Taken together, it is suggested that EEP can safely be utilised in the prevention of psoriasis-related inflammatory changes without causing any toxic effect.

Topics: Animals; Anti-Infective Agents; Antioxidants; Disulfides; Drug Evaluation, Preclinical; Glutathione; Lipid Peroxidation; Macrophages; Male; Mice; Micronucleus Tests; Oxidative Stress; Peritonitis; Propolis; Psoriasis; Salicylates; Thermography

Bornyl salicylate (BS) is a salicylic derivative, obtained by sterification of salicylic acid and monoterpene (-)-borneol, and its topical use in inflammatory diseases was described in the early 20th century. It is also known that borneol presents neuroprotective, genoprotective and analgesic properties. The purpose of this study was to evaluate BS in experimental models of acute inflammation. The toxicity of BS was analyzed by measuring water and food intake, weight, mortality and weight of main organs. To assess its anti-inflammatory effect, BS-treated mice were challenged with carrageenan, prostaglandin E2 (PGE2), bradikynin (BK) or histamine (HIS)-induced paw edema, zymosan-induced peritonitis and vascular permeability induced by acetic acid. Nitric oxide (NO) production was analyzed in peritoneal macrophage cultures. There was no sign of acute toxicity of BS in male and female mice. Furthermore, treatment with BS was significantly (p < 0.05) effective in reducing paw edema induced by carrageenan in early and late phases; this effect was related to PGE2 and BK, but HIS independent. Neutrophil migration and cytokine release (TNF-α, IL-1β and IL-6) induced by zymosan and fluid leakage induced by acetic acid were also reduced in BS-treated animals. In vitro, BS (10 µg/mL) reduced NO production in LPS-stimulated macrophages. These data suggest that BS has an anti-inflammatory effect, which is related, at least in part, with decrease of mediators as PGE2, NO and pro-inflammatory cytokines. However, further studies should be done to explore its potential as an anti-inflammatory drug.

Topics: Animals; Anti-Inflammatory Agents, Non-Steroidal; Bradykinin; Carrageenan; Cell Movement; Cytokines; Dinoprostone; Drug Evaluation, Preclinical; Eating; Female; Lipopolysaccharides; Macrophages, Peritoneal; Male; Mice; Neutrophils; Nitric Oxide; Oxytocics; Peritonitis; Salicylates; Vasodilator Agents; Zymosan

Topics: Animals; Female; Intestinal Perforation; Lung; Male; Methylprednisolone; Methylprednisolone Hemisuccinate; Peritonitis; Rats; Rats, Inbred Strains; Respiratory Distress Syndrome; Salicylates

Topics: Adrenalectomy; Aminopyrine; Animals; Anti-Inflammatory Agents; Capillary Permeability; Edema; Fever; Granuloma; Guinea Pigs; Hydrocortisone; Male; Mice; Morphine; Peritonitis; Phenylbutazone; Pyridines; Rabbits; Rats; Salicylates; Wound Healing

Topics: Blood Transfusion; Burns; Child; Chlorides; Humans; Infant; Infant, Newborn; Infant, Newborn, Diseases; Infusions, Parenteral; Intestinal Obstruction; Kidney; Peritonitis; Potassium; Pyloric Stenosis; Salicylates; Sodium; Toxicology; Water; Water-Electrolyte Balance