salicylates and Mouth-Neoplasms

Carboplatin (CBP)-resistant cell line (Tca8113/CBP) and pingyangmycin (PYM)-resistant cell line (Tca8113/PYM) were established in vitro. Ginkgolic acids' influence over multidrug resistance (MDR) of drug-resistant cells was discussed by ginkgolic acids coupled with chemotherapy drugs.. The expression of P-glycoprotein (P-gp) was detected by immunohistochemistry. MTT assay was applied to ascertain the resistance index of drug-resistant cells. The effect of different concentrations of ginkgolic acids on the proliferation of drug-resistant cells and parental cell was measured by MTT assay. Making sure the non-toxic concentration of ginkgolic acids and observing the reversal effect of ginkgolic acids on drug-resistant cells. Resistance index was redetermined by MTT assay after ginkgolic acids coupled with chemotherapy drugs induced the cell lines for some time.. Immunohistochemistry showed that P-gp positive expression rate of drug-resistant cells was significantly higher than parental cells. The non-toxic concentration of ginkgolic acids which was determined by MTT assay was 10 microg x mL(-1). The reversal folds of Tca8113/CBP cell line to CBP and Tca8113/PYM cell line to PYM were 2.94 and 2.43 respectively. Before coupled with ginkgolic acids, the resistance indices of Tca8113/CBP and Tca8113/PYM cell lines were 3.24 and 11.9 respectively. When ginkgolic acids was added with chemotherapy drugs for some time, the resistance indices of Tca8113/CBP and Tca8113/PYM cell lines were 2.18 and 4.43 respectively.. This experiment successfully induced the drug-resistant cell lines of Tca8113/CBP and Tca8113/PYM. The method of chemotherapy drugs coupled with ginkgolic acids further confirmed the effect on proliferation of Tca8113/CBP and Tca8113/PYM cell lines was reducing. Non-toxic concentration of ginkgolic acids can partially reverse the drug resistance of Tca8113/ CBP and Tca8113/PYM cell lines. Furthermore, MDR level of drug-resistant cells decreased somewhat when they were induced by ginkgolic acids coupled with chemotherapy drugs for some time.

Topics: Antineoplastic Agents; ATP Binding Cassette Transporter, Subfamily B, Member 1; Bleomycin; Carcinoma, Squamous Cell; Drug Resistance, Multiple; Drug Resistance, Neoplasm; Humans; Mouth Neoplasms; Salicylates

There are three types of the root canal sealers commonly used in clinical applications. They are calcium hydroxide base (Sealapex), zinc oxide-eugenol base (Canals), and epoxy-resin base (AH Plus). Elutable substances and degradation products from root canal sealers may gain access to periodontal tissue in a number of ways. The purpose of the present study was to evaluate the biologic effects of the root canal sealers on human oral cancer cell line (OC2). The tetrazolium bromide (MTT) assay was to evaluate the cell's survival rate. The DNA electrophoresis was used to evaluate the OC2 cell's DNA damage. The results demonstrated that the above root canal sealers' survival rates are in dose-dependent increase (p < 0.05). The toxicity of fresh mix group is higher than that of the mixed after 24h group. DNA fragmentation assay of sealer treated OC2 cells shows a smear layer pattern on the electrophoresis gel. There is no DNA damage found. The toxicity that regulated the cell death is not by the apoptic change of cells.

Topics: Apoptosis; Calcium Hydroxide; Cell Line, Tumor; Cell Survival; DNA Fragmentation; Humans; Mouth Neoplasms; Necrosis; Pit and Fissure Sealants; Root Canal Filling Materials; Salicylates

Three main types of root canal sealer are currently commonly used in pulp treatment: zinc oxide eugenol-based, calcium hydroxide-based, and epoxy resin-based sealers. In the present study, the genotoxicity of sealer on oral carcinoma cells was evaluated by single-cell gel electrophoresis assay (comet assay). The whole length of the comet and the diameter of the head were measured using an image analysis system. The results were analyzed by one-way analysis of variance to compare the various means. The zinc oxide eugenol-based sealers (Canals, Canals-N, and Tubilseal) did not always cause a dose-dependent increase in genotoxicity. The resin-based sealers (Topseal, AH 26, and AH Plus) caused a dose-dependent increase in genotoxicity, but no such effect was seen with the calcium hydroxide-based sealer (Sealapex). The highest level of DNA damage was induced by the resin-based sealers.

Topics: Analysis of Variance; Bismuth; Calcium Hydroxide; Carcinoma, Squamous Cell; Comet Assay; DNA Damage; Drug Combinations; Epoxy Resins; Humans; Methenamine; Mouth Neoplasms; Root Canal Filling Materials; Salicylates; Silver; Titanium; Tumor Cells, Cultured; Zinc Oxide-Eugenol Cement

Topics: Drug Combinations; Drug Labeling; Drug Packaging; Humans; Mouth Neoplasms; Mouthwashes; Salicylates; Terpenes

A 23-year-old white female with a history of psychological instability presented with burning, adhesive, white plaques of the buccal mucosa. She had been unsuccessfully treated for 1.5 years with topical retinoids as well as topical and systemic corticosteroids. The differential diagnoses included leukoplakia, cheek biting and lichen planus. Clinical and histopathological examinations suggested the diagnosis of severe oral leukoedema. Careful history and clandestine surveillance of the patient finally revealed the lesions to be due to a caustic (over-the-counter product for the treatment of corns), which the patient regularly applied to her buccal mucosa.

Topics: Adult; Diagnosis, Differential; Drug Combinations; Edema; Factitious Disorders; Female; Humans; Lactates; Lactic Acid; Leukoplakia, Oral; Mouth Mucosa; Mouth Neoplasms; Salicylates

Topics: Adult; Aged; Diflunisal; Female; Humans; Male; Mandibular Neoplasms; Maxillary Neoplasms; Middle Aged; Mouth Neoplasms; Pain, Postoperative; Salicylates

Topics: Animals; Carcinoma, Squamous Cell; Drug Interactions; Female; Hematopoiesis; Humans; Methotrexate; Mice; Mouth Neoplasms; Salicylates

Topics: Anti-Infective Agents, Local; Coal Tar; Glycerol; Humans; Mouth Neoplasms; Propylene Glycol; Root Canal Therapy; Salicylates; Salicylic Acid; Trichloroacetic Acid

Topics: Anti-Infective Agents, Local; Coal Tar; Glycerol; Humans; Mouth Neoplasms; Propylene Glycol; Root Canal Therapy; Salicylates; Salicylic Acid; Trichloroacetic Acid