safranine-t and Hypertrophy

The temporomandibular joint (TMJ) cartilage consists of condylar cartilage and disc and undergoes continuous remodeling throughout post-natal life. To maintain the integrity of the TMJ cartilage, anti-angiogenic factors play an important role during the remodeling process. In this study, we investigated the expression of the anti-angiogenic factor, chondromodulin-1 (ChM-1), in TMJ cartilage and evaluate its potential role in TMJ remodeling.. Eight TMJ specimens were collected from six 4-month-old Japanese white rabbits. Safranin-O staining was performed to determine proteoglycan content. ChM-1 expression in TMJ condylar cartilage and disc was determined by immunohistochemistry. Three human perforated disc tissue samples were collected for investigation of ChM-1 and vascular endothelial growth factor (VEGF) distribution in perforated TMJ disc.. Safranin-O stained weakly in TMJ compared with tibial articular and epiphyseal cartilage. In TMJ, ChM-1 was expressed in the proliferative and hypertrophic zone of condylar cartilage and chondrocyte-like cells in the disc. No expression of ChM-1 was observed in osteoblasts and subchondral bone. ChM-1 and VEGF were both similarly expressed in perforated disc tissues.. ChM-1 may play a role in the regulation of TMJ remodeling by preventing blood vessel invasion of the cartilage, thereby maintaining condylar cartilage and disc integrity.

Topics: Angiogenesis Inhibitors; Animals; Bone Remodeling; Cartilage, Articular; Cell Proliferation; Chondrocytes; Coloring Agents; Epiphyses; Female; Humans; Hypertrophy; Intercellular Signaling Peptides and Proteins; Mandibular Condyle; Membrane Proteins; Osteoblasts; Phenazines; Proteoglycans; Rabbits; Rats; Rats, Inbred Lew; Temporomandibular Joint; Temporomandibular Joint Disc; Temporomandibular Joint Disorders; Tibia; Vascular Endothelial Growth Factor A