safranine-t has been researched along with AIDS-Related-Opportunistic-Infections* in 2 studies
2 other study(ies) available for safranine-t and AIDS-Related-Opportunistic-Infections
| Article | Year |
|---|---|
In vitro effects of micafungin against Candida biofilms on polystyrene and central venous catheter sections.
Long-term inserted and surgically implanted catheters can be colonised by Candida spp. Candida biofilms in vitro are often resistant to antifungal agents. The aim of this study was to investigate the in vitro activity of micafungin (MFG) against six Candida spp. biofilms on polystyrene (PS) and central venous catheter (CVC) sections. Safranin staining and differential interference contrast microscopy were used to demonstrate biofilm production. MFG activity was determined by the reduction in metabolic activity (%RMA) by tetrazolium reduction assay on both substrates. In vitro, Candida albicans, Candida parapsilosis, Candida glabrata, Candida tropicalis, Candida dubliniensis and Candida kefyr produced mature biofilms on PS and CVC sections. MFG was active against C. kefyr (0.5 microg/mL) and C. glabrata (<0.5 microg/mL) on PS. However, MFG displayed resistance (>16 microg/mL) against C. albicans, C. dubliniensis,C. tropicalis and C. parapsilosis. On CVC disks, MFG was active against C. glabrata (1 microg/mL) as well as C. parapsilosis and C. albicans (<0.5 microg/mL). MFG was resistant (>16 microg/mL) against C. dubliniensis, C. tropicalis and C. kefyr. MFG was active in vitro against all six Candida spp. on both substrates. However, MFG could not reduce the metabolic activity completely even at the highest concentration. Topics: Adult; AIDS-Related Opportunistic Infections; Antifungal Agents; Biofilms; Candida; Candidiasis; Catheterization, Central Venous; Catheters, Indwelling; Child; Echinocandins; Humans; Infant, Newborn; Infant, Premature, Diseases; Lipopeptides; Lipoproteins; Micafungin; Microbial Sensitivity Tests; Microscopy, Interference; Peptides, Cyclic; Phenazines; Polystyrenes; Staining and Labeling; Tetrazolium Salts | 2006 |
Uniform staining of Cyclospora oocysts in fecal smears by a modified safranin technique with microwave heating.
Cyclospora, a coccidian protist, is increasingly being identified as an important, newly emerging parasite that causes diarrhea, flatulence, fatigue, and abdominal pain leading to weight loss in immunocompetent persons with or without a recent travel history as well as in patients with AIDS. Modified Kinyoun's acid-fast stain is the most commonly used stain to identify the oocyst of this parasite in fecal smears. Oocysts of Cyclospora stain variably by the modified acid-fast procedure, resulting in the possible misidentification of this parasite. We examined fecal smears stained by six different procedures that included Giemsa, trichrome, chromotrope, Gram-chromotrope, acid-fast, and safranin stains. We report on safranin-based stain that uniformly stains oocysts of Cyclospora a brilliant reddish orange, provided that the fecal smears are heated in a microwave oven prior to staining. This staining procedure, besides being superior to acid-fast staining, is fast, reliable, and easy to perform in most clinical laboratories. Topics: AIDS-Related Opportunistic Infections; Animals; Coccidiosis; Coloring Agents; Eucoccidiida; Evaluation Studies as Topic; Feces; Hot Temperature; Humans; Microwaves; Parasitology; Phenazines; Staining and Labeling | 1997 |