rs-39604 and Feeding-and-Eating-Disorders

To study the functional contributions of the 5-HT4 receptor subtype of serotonin (5-HT), we have generated knockout mice lacking the 5-HT4 receptor gene. The male mutant mice exhibit a hyposensitivity to anorexic stress. Our recent data indicate that the pharmacological inactivation, using a systemic injection of the 5-HT4 receptor antagonist RS39604 (0.5 mg/kg), suppressed restraint stress-induced anorexia in wild-type female mice. In parallel, the same treatment reduced the 3,4-N-methylenedioxymethamphetamine (" ecstasy", 10 mg/kg)-induced anorexia in male wild-type mice. Our neurochemical analyses suggest that the mechanisms underlying feeding disorders in 5-HT4 receptor knockout mice are related to a lesser efficacy of 5-HT (hypothalamus, nucleus accumbens), leptin and the cocaine-amphetamine related transcript to reduce food intake following stress.

Topics: Animals; Anorexia; Appetite; Chromatography, High Pressure Liquid; Corticosterone; Feeding and Eating Disorders; Gene Expression Regulation; Leptin; Limbic System; Male; Mice; Mice, Knockout; N-Methyl-3,4-methylenedioxyamphetamine; Nerve Tissue Proteins; Piperidines; Propane; Receptors, Serotonin, 5-HT4; Restraint, Physical; Reverse Transcriptase Polymerase Chain Reaction; Serotonin; Serotonin 5-HT4 Receptor Antagonists; Serotonin Antagonists; Stress, Psychological