roussin-s-black-salt and Neoplasms

Peroxynitrite (ONOO

Topics: A549 Cells; Animals; Humans; Iron Compounds; Lithium Compounds; Mice, Nude; Nanocomposites; Neoplasm Transplantation; Neoplasms; Nitroso Compounds; Peroxynitrous Acid; Radiation-Sensitizing Agents; X-Rays

A facile and general coordination-precipitation method is developed to synthesize insoluble metal Roussin's black salts (Me-RBSs) as a new type of NIR-responsive NORMs. The weak-field ligand coordination of metal

Topics: Antineoplastic Agents; Drug Delivery Systems; Humans; Infrared Rays; Iron Compounds; Metals; Neoplasms; Nitric Oxide; Nitroso Compounds; Salts

A metal-nitrosyl complex, Roussin's black salt (RBS), releases nitric oxide after illumination. Approximately 3.7 NO molecules were released from one RBS molecule. Both short- and long-term effects of photogenerated NO on the two neoplastic cell lines: human (SK-MEL188) and mouse (S91) have been investigated. Exogenous NO from RBS was toxic to cells in a dose-dependent manner. Apoptotic damage predominates in the response to the injury, as shown by TUNEL assay. NO and its short-lived metabolites, but not other RBS photoproducts, are responsible for cellular death. RBS in dark was toxic to cells at concentrations above 1 microM. This relatively high cytotoxicity of RBS in the dark prevents its application as a systemic anticancer agent in vivo, unless it is applied locally.

Topics: Animals; Apoptosis; Cell Line, Tumor; Dermatitis, Phototoxic; Dose-Response Relationship, Drug; Humans; Iron Compounds; Melanoma; Mice; Neoplasms; Nitric Oxide; Nitric Oxide Donors; Nitroso Compounds