rosmarinic-acid and Hemolysis

rosmarinic-acid has been researched along with Hemolysis* in 10 studies

Other Studies

10 other study(ies) available for rosmarinic-acid and Hemolysis

ArticleYear
Hydrophobicity and antioxidant activity acting together for the beneficial health properties of nordihydroguaiaretic acid.
    Food & function, 2015, Volume: 6, Issue:6

    Nordihydroguaiaretic acid (NDGA) and rosmarinic acid (RA), phenolic compounds found in various plants and functional foods, have known antioxidant and anti-inflammatory properties. In the present study, we comparatively investigated the importance of hydrophobicity and oxidisability of NDGA and RA, regarding their antioxidant and pharmacological activities. Using a panel of cell-free antioxidant protocols, including electrochemical measurements, we demonstrated that the anti-radical capacities of RA and NDGA were similar. However, the relative capacity of NDGA as an inhibitor of NADPH oxidase (ex vivo assays) was significantly higher compared to RA. The inhibitory effect on NADPH oxidase was not related to simple scavengers of superoxide anions, as confirmed by oxygen consumption by the activated neutrophils. The higher hydrophobicity of NDGA was also a determinant for the higher efficacy of NDGA regarding the inhibition of the release of hypochlorous acid by PMA-activated neutrophil and cytokine (TNF-α and IL-10) production by Staphylococcus aureus-stimulated peripheral blood mononuclear cells. In conclusion, although there have been extensive studies about the pharmacological properties of NDGA, our study showed, for the first time, the importance not only of its antioxidant activity, but also its hydrophobicity as a crucial factor for pharmacological action.

    Topics: Adult; Anti-Inflammatory Agents, Non-Steroidal; Antioxidants; Cells, Cultured; Cinnamates; Cytokines; Depsides; Enzyme Inhibitors; Hematologic Agents; Hemolysis; Humans; Hydrophobic and Hydrophilic Interactions; Hypochlorous Acid; Leukocytes, Mononuclear; Lymphocyte Activation; Masoprocol; NADPH Oxidases; Neutrophil Activation; Neutrophils; Osmolar Concentration; Rosmarinic Acid; Young Adult

2015
Inhibition effects of isolated compounds from Artemisia rubripes Nakai of the classical pathway on the complement system.
    Immunopharmacology and immunotoxicology, 2012, Volume: 34, Issue:2

    The study evaluated the anticomplement activity from isolated compounds from Artemisia rubripes Nakai from South Korea on the classical pathway. In the previous works, Artemisia rubripes chloroform extracts showed inhibitory activity against complement system. The chromatographic separation of a chloroform chloride extract of Artemisia rubripes led to the isolation of three compounds. Their structures were characterized to be scopoletin (1), 11,(13)-triene-6,12-olide (2), and 1β,6α-dihydroxy-4(15)-eudesmene (3) by spectroscopic data. This is the first report of anticomplement activity of isolated compounds from Artemisia rubripes.

    Topics: Animals; Artemisia; Chloroform; Chromatography, High Pressure Liquid; Chromatography, Thin Layer; Cinnamates; Complement Inactivating Agents; Complement Pathway, Classical; Depsides; Flavonoids; Hemolysis; Inhibitory Concentration 50; Molecular Structure; Plant Extracts; Rabbits; Rosmarinic Acid; Scopoletin; Sesquiterpenes; Sesquiterpenes, Eudesmane; Sheep

2012
Isolated compounds from Sorghum bicolor L. inhibit the classical pathway of the complement.
    Immunopharmacology and immunotoxicology, 2012, Volume: 34, Issue:2

    The present study evaluated the anticomplement effects from isolated compounds of Sorghum bicolor in classical pathway complement system. Using column chromatograph, three compounds; Sorgoleone-362 (1), Sorgoleone-360 (2) and Sorgoleone-386 (3) were isolated and evaluated for in vitro anticomplement activity. Sorgoleone-386 showed inhibitory activity against complement system with 50% inhibitory concentrations (IC(50)) values of 148.3μg/ml. This is the first report of anticomplement activity of isolated compounds from Sorghum bicolor.

    Topics: Animals; Benzoquinones; Chromatography, High Pressure Liquid; Chromatography, Thin Layer; Cinnamates; Complement Inactivating Agents; Complement Pathway, Classical; Depsides; Flavonoids; Hemolysis; Inhibitory Concentration 50; Lipids; Molecular Structure; Plant Extracts; Plant Roots; Rabbits; Rosmarinic Acid; Sheep; Sorghum

2012
Anticomplement activity of polyacetylenes from leaves of Dendropanax morbifera Leveille.
    Phytotherapy research : PTR, 2011, Volume: 25, Issue:5

    The present study evaluated the anticomplement effect of polyacetylenes from Dendropanax morbifera (Araliaceae) in the classical pathway complement system. The leaves of D. morbifera were evaluated with regard to its anticomplement activity, and its active principles identified following activity-guided isolation. An aqueous CCl(4) fraction of the leaves of D. morbifera exhibited significant anticomplement activity on the classical pathway complement system, which was expressed as total hemolytic activity. Three polyacetylenes isolated from the leaves of D. morbifera, namely (3S)-falcarinol (1), (3S,8S)-falcarindiol (2) and (3S)-diynene (3). Compounds 1, 2 and 3 showed inhibitory activity against complement system with 50% inhibitory concentrations (IC(50)) values of 87.3 µM, 15.2 µM and 39.8 µM. Among the compounds tested, 2 showed the most potent anticomplement activity (IC(50), 15.2 µM).

    Topics: Animals; Araliaceae; Cinnamates; Complement Inactivating Agents; Complement Inactivator Proteins; Complement Pathway, Classical; Depsides; Diynes; Erythrocytes; Fatty Alcohols; Flavonoids; Hemolysis; Humans; Inhibitory Concentration 50; Male; Plant Extracts; Plant Leaves; Polyynes; Rabbits; Rosmarinic Acid; Sheep; Young Adult

2011
Curcumin inhibits the classical and the alternate pathways of complement activation.
    Annals of the New York Academy of Sciences, 2005, Volume: 1056

    Curcumin (Cur), the golden yellow phenolic compound in turmeric, is well studied for its medicinal properties. In the current investigation, Cur dissolved using sodium hydroxide solution (CurNa) was tested for in vitro complement inhibitory activity and compared with rosmarinic acid (RA) and quercetin (Qur) dissolved using sodium hydroxide (RANa and QurNa, respectively) and the vaccinia virus complement control protein (VCP). The comparative study indicated that CurNa inhibited the classical complement pathway dose dependently (IC50 = 404 microM). CurNa was more active than RANa, but less active than QurNa. VCP was about 2,212, 2,786, and 4,520 times more active than QurNa, CurNa, and RANa, respectively. Further study revealed that CurNa dose dependently inhibited zymosan-induced activation of the alternate pathway of complement activation.

    Topics: Analysis of Variance; Cinnamates; Complement Pathway, Alternative; Complement Pathway, Classical; Curcumin; Depsides; Hemolysis; Humans; Quercetin; Rosmarinic Acid; Viral Proteins

2005
Biological evaluation of proanthocyanidin dimers and related polyphenols.
    Journal of natural products, 1999, Volume: 62, Issue:7

    A series of dimeric procyanidins (1-9) and some related polyphenols (10-15) were chosen as model compounds in a comparative investigation for various biological activities in order to obtain structure-activity relationships. Antiviral [herpes simplex virus (HSV) and human immunodeficiency virus (HIV)], antibacterial, superoxide radical-scavenging, and complement-modulating properties were assessed. In general, more pronounced activities were seen with epicatechin-containing dimers for anti-HSV, anti-HIV, and radical-scavenging effects, while the presence of ortho-trihydroxyl groups in the B-ring was important in compounds exhibiting anti-HSV and radical-scavenging effects and complement classical pathway inhibition. Double interflavan linkages gave rise to interesting antiviral effects (HSV and HIV) and complement inhibition. The influence of the degree of polymerization or the type of interflavan linkage (4-->6 or 4-->8) differed in the different biological systems evaluated. Only minor or moderate antibacterial effects were observed for the compounds under investigation.

    Topics: Anthocyanins; Anti-Bacterial Agents; Anti-HIV Agents; Anti-Infective Agents; Antiviral Agents; Bacteria; Complement Inactivator Proteins; Euphorbiaceae; Flavonoids; Free Radical Scavengers; Hemolysis; Magnetic Resonance Spectroscopy; Microbial Sensitivity Tests; Peru; Phenols; Plants, Medicinal; Polymers; Simplexvirus

1999
Inhibition of complement by covalent attachment of rosmarinic acid to activated C3b.
    Biochemical pharmacology, 1999, Jun-15, Volume: 57, Issue:12

    Rosmarinic acid has been reported to inhibit complement activation in vivo as well as in vitro. Previous studies suggested that the inhibitory effect was due to inhibition of C3/C5 convertases, but inhibition of C3b attachment would yield the same results. Recent work in our laboratory demonstrated that compounds with polyhydroxylated phenyl rings are highly reactive with the thioester bond in nascent C3b. These compounds block complement activation by preventing attachment of C3b to the activating surface. Because rosmarinic acid contains two 3,4-dihydroxyphenyl groups, the current study was undertaken to re-examine the mechanism of inhibition by analyzing the effect of rosmarinic acid on C3b attachment. In assays using purified complement proteins, rosmarinic acid inhibited covalent attachment of C3b to cells with an 1C50 = 34 microM. Inhibition of C5 convertase activity required 1500 microM rosmarinic acid, and no significant inhibition of the C3 convertase enzyme, which produces C3b from C3, was observed at 10,000 microM. In hemolytic assays using human serum, rosmarinic acid was shown to inhibit activation of both the classical (IC50 = 180 microM) and the alternative (IC50 = 160 microM) pathways of complement. Rosmarinic acid concentrations up to 10,000 microM did not cause direct inactivation of C3. Radioiodination of rosmarinic acid was used to demonstrate covalent activation-dependent incorporation of rosmarinic acid specifically into the thioester-containing alpha'-chain of nascent C3b. These findings indicate that inhibition of complement activation by rosmarinic acid is due to the reaction of rosmarinic acid with the activated thioester of metastable C3b, resulting in covalent attachment of the inhibitor to the protein.

    Topics: Animals; Cinnamates; Complement Activation; Complement C3; Complement C3-C5 Convertases; Complement C3b; Complement C3b Inactivator Proteins; Depsides; Erythrocytes; Hemolysis; Humans; Rosmarinic Acid; Sheep

1999
In vitro anticomplementary activity of constituents from Morinda morindoides.
    Journal of natural products, 1995, Volume: 58, Issue:3

    In a screening program for complement classical pathway modulation, an 80% MeOH extract of the leaves of Morinda morindoides showed potent dose-dependent anticomplementary activity. Bioassay-guided chromatographic separation of the active constituents led to the isolation of ten flavonoids of which two were aglycones. The compounds were tested in vitro for their putative complement-inhibiting properties on the classical (CP) and the alternative (AP) pathways of the complement system. The results indicated that quercetin [1], quercetin 3-O-rhamnoside (quercitrin) [5], and quercetin 3-O-rutinoside (rutin) [7] showed similar anticomplementary activities (inhibition) on the CP of complement. A mixture of two kaempferol triglycosides isolated and denoted as M(015), also had a good inhibitory effect. The effects of these compounds were dose-dependent for this pathway. On the AP of complement, quercetin [1] and M(015) had, respectively, more pronounced inhibitory and activatory effects than the other tested flavonoids, but their effects were not dose-dependent for this pathway. The other isolated flavonoids showed weak effects or were inactive for both pathways.

    Topics: Animals; Complement Inactivator Proteins; Complement Pathway, Alternative; Complement Pathway, Classical; Flavonoids; Guinea Pigs; Hemolysis; Humans; In Vitro Techniques; Plant Extracts; Plant Leaves; Plants, Medicinal; Rabbits; Sheep

1995
Complement-dependent stimulation of prostacyclin biosynthesis: inhibition by rosmarinic acid.
    Biochemical pharmacology, 1986, Apr-15, Volume: 35, Issue:8

    Topics: Animals; Anti-Inflammatory Agents; Cinnamates; Complement C3-C5 Convertases; Complement System Proteins; Depsides; Elapid Venoms; Epoprostenol; Hemolysis; Humans; Peritoneum; Rabbits; Rosmarinic Acid

1986
Modification of endotoxin-induced haemodynamic and haematological changes in the rabbit by methylprednisolone, F(ab')2 fragments and rosmarinic acid.
    British journal of pharmacology, 1985, Volume: 84, Issue:2

    The effects of methylprednisolone, F(ab')2 fragments of human gamma globulins and rosmarinic acid, an inhibitor of complement activation, were tested on endotoxin-induced haemodynamic and haematological changes in the rabbit. Their effects were compared with complement depletion by cobra venom factor (CVF) pretreatment. The results provide further evidence for the role of complement activation and the concomitant triggering of the arachidonic acid cascade in the early phase of shock. The formation of vasoactive prostanoids (prostacyclin and thromboxane A2), the arterial hypotension and the thrombocytopenia were largely dependent on the presence of the intact complement system. F(ab')2 fragments (150 mg kg-1, i.v.) diminished the second fall in blood pressure to some extent but failed to alter any of the other endotoxin-induced changes. Methylprednisolone (40 mg kg-1, i.v.) given 10 min before endotoxin significantly reduced the activation of complement, the second rise of prostacyclin and the secondary hypotension, but was without effect on the early thromboxane peak of the haematological features of endotoxin shock. Rosmarinic acid (20 mg kg-1, i.v.) may be of potential interest for treatment of septic shock, since the drug suppressed the endotoxin-induced activation of complement, the formation of prostacyclin, both hypotensive phases, the thrombocytopenia and the concomitant release of thromboxane A2. The role of leukocytes and their arachidonic acid metabolites in plasma exudation deserves further investigation, because leukopenia and pulmonary oedema were not complement-dependent and were not affected by any of the treatments. Our results indicate that drugs, interfering with complement activation and/or prostaglandin biosynthesis, may be beneficial in endotoxin shock, provided that they are administered at an early stage.

    Topics: 6-Ketoprostaglandin F1 alpha; Animals; Blood Pressure; Cinnamates; Complement Activation; Depsides; Elapid Venoms; Endotoxins; Hemodynamics; Hemolysis; Immunoglobulin Fab Fragments; In Vitro Techniques; Leukocyte Count; Methylprednisolone; Platelet Count; Pulmonary Edema; Rabbits; Rosmarinic Acid; Thromboxane B2

1985