rosmarinic-acid and Cell-Transformation--Neoplastic

Six compounds related to pyrrolizidine alkaloids have been subjected to an in vitro mammalian cell transformation test. Two hepatocarcinogenic alkaloids (retrorsine and monocrotaline) and one synthetic analogue (synthanecine A bis-N-ethylcarbamate) gave positive results while a non-toxic alkaloid (rosmarinine) was negative in the test. Positive results were also given by dehydroretronecine, a secondary pyrrolic alkaloid metabolite, and the closely related synthetic compound 2,3-bishydroxymethyl-1-methylpyrrole. These observations lend support to the hypothesis that a simple alkylating pyrrole is the biologically active chemical agent derived from these alkaloids. The negative transformation response observed for the non-carcinogenic alkaloid rosmarinine establishes that the carcinogenic alkaloids are inducing transformation rather than simply selecting for spontaneous transformants. The mammalian-derived cells used in this study, unlike S. typhimurium, were capable of activating retrorsine in the absence of an auxiliary source of metabolising enzymes (S-9 mix).

Topics: Animals; Antineoplastic Agents, Phytogenic; Antioxidants; Carcinogens; Cell Nucleus; Cell Transformation, Neoplastic; Cinnamates; Depsides; In Vitro Techniques; Liver; Male; Mitochondria, Liver; Monocrotaline; Pyrrolizidine Alkaloids; Rats; Rats, Sprague-Dawley; Rosmarinic Acid