rosin and Hypersensitivity

Topics: Humans; Hypersensitivity; Patch Tests; Resins, Plant; Terpenes

Topics: Asthma; Humans; Hypersensitivity; Occupational Diseases; Occupational Exposure; Resins, Plant

The potential of colophony fumes from soldering flux to induce asthma has been known since the 1970s, however, no direct in vitro or in vivo evidence has been reported. The present study investigated the potential of colophony to stimulate human phagocytic cells to produce reactive oxygen species.. The human cell line HL-60 was differentiated to produce cells with a monocyte-like and a neutrophil-like phenotypes. A number of procedures were used to confirm the phenotype of these differentiated cells including morphology, esterase activity, flow cytometry and phagocytosis. The potential of colophony to stimulate human phagocytic cells to produce reactive oxygen species was monitored using flow cytoenzymology.. We were able to show that intracellular peroxide levels were increased in both monocyte-like and neutrophil-like cells, but not in undifferentiated HL-60 cells following the addition of colophony.. The resin acid epoxides and hydroperoxides which have been suggested to be sensitizers in contact allergy, are degraded during the soldering process. However, conditions for the oxidation of colophony may occur in vivo as a result of the colophony-induced oxidative burst from neutrophils and monocytes. These oxidation products may then interact with body proteins to further initiate immune responses. Therefore for the preparation of low molecular weight chemical (LMWC)-protein conjugates, consideration must be taken to determine whether the LMWC is undergoing a reaction in vivo before it is interacting with body proteins.

Topics: Cell Differentiation; Cell Survival; Esterases; Flow Cytometry; HL-60 Cells; Humans; Hypersensitivity; Monocytes; Neutrophils; Phagocytosis; Reactive Oxygen Species; Resins, Plant; Respiratory Burst

The aim of the present investigation was to obtain information on the repertoire of patient-related remedies and materials used by dental hygienists and on the frequency and nature of side effects observed among their patients. Norwegian hygienists received a questionnaire on dental remedies and materials used, the number of patients seen, and side effects observed during the past year and earlier. Information from 169 hygienists provided a list of remedies comprising fluoride-containing varnishes, polishing remedies, pit and fissure sealants, oral disinfectants, fluoride gels and solutions, and plaque-disclosing solutions. In addition, some hygienists used temporary dental materials, polyalkeonate/composites, and local anesthetic spray or ointment. Fourteen brands of latex gloves were used. Possible side effects of general and local nature were observed by 37 dental hygienists. Reactions associated with the application of Duraphat in children (> 1:1640) and contact with latex gloves in adult patients (> 1:3300) were most important. The findings are discussed with specific emphasis on the content of colophony in the fluoride varnish.

Topics: Adolescent; Adult; Aged; Child; Dental Hygienists; Dental Materials; Dental Polishing; Dental Prophylaxis; Fluorides, Topical; Gloves, Surgical; Humans; Hypersensitivity; Norway; Occupational Diseases; Resins, Plant; Rubber; Sodium Fluoride; Tars