rocaglamide and Cell-Transformation--Neoplastic

The ribosome is centrally situated to sense metabolic states, but whether its activity, in turn, coherently rewires transcriptional responses is unknown. Here, through integrated chemical-genetic analyses, we found that a dominant transcriptional effect of blocking protein translation in cancer cells was inactivation of heat shock factor 1 (HSF1), a multifaceted transcriptional regulator of the heat-shock response and many other cellular processes essential for anabolic metabolism, cellular proliferation, and tumorigenesis. These analyses linked translational flux to the regulation of HSF1 transcriptional activity and to the modulation of energy metabolism. Targeting this link with translation initiation inhibitors such as rocaglates deprived cancer cells of their energy and chaperone armamentarium and selectively impaired the proliferation of both malignant and premalignant cells with early-stage oncogenic lesions.

Topics: Animals; Antineoplastic Agents; Benzofurans; Cell Line, Tumor; Cell Proliferation; Cell Transformation, Neoplastic; DNA-Binding Proteins; Energy Metabolism; Gene Expression Regulation, Neoplastic; Heat Shock Transcription Factors; High-Throughput Screening Assays; Humans; Mice; Neoplasm Transplantation; Neoplasms; NIH 3T3 Cells; Protein Biosynthesis; Ribosomes; Transcription Factors