ro13-9904 and Diarrhea--Infantile

This study aimed to trace the transmission source of Salmonella enterica serovar Typhimurium and Salmonella enterica serovar Enteritidis strains associated with enteric infections in Shanghainese children, and understand the molecular mechanism of resistance to third-generation cephalosporins and ciprofloxacin.. The profiles of pulsed-field gel electrophoresis (PFGE) were compared among the isolates from children, animal, and environment. Antimicrobial susceptibility was determined using the minimal inhibitory concentrations and Kirby-Bauer disk diffusion method. Extended-spectrum β-lactamase (ESBL) producing isolates mediated by resistance genes were identified using polymerase chain reaction and sequencing.. Based on PFGE patterns, 49 (33.1%) of 148 human Salmonella Typhimurium isolates located in the dominant PFGE clusters were genetically related to the isolates from poultry source, environment water, aquatic products, and reptiles, whereas 97 (97.0%) of 100 human Salmonella Enteritidis isolates were genetically related to isolates from poultry and water. The rates of resistance to ceftriaxone among clinical Salmonella Typhimurium and Salmonella Enteritidis isolates were 42.0% and 14.2%, respectively. Besides, 35.1% of Salmonella Typhimurium isolates displayed resistance to ciprofloxacin; 64.9% of Salmonella Typhimurium isolates and 97.0% of Salmonella Enteritidis isolates displayed reduced susceptibility to ciprofloxacin. Of 64 ESBL/AmpC-producing strains, CTX-M, TEM, DHA, and CMY were detected at frequencies of 86.0%, 62.5%, 7.8%, 3.1%, and 3.1%, respectively.. The transmission sources of Salmonella Typhimurium and Salmonella Enteritidis infections in Shanghainese children were diverse. The high prevalence of resistance to third-generation cephalosporins and ciprofloxacin mediated by multiple molecular mechanisms needs continuous monitoring and intervention.

Topics: Anti-Bacterial Agents; Ceftriaxone; Child; Child Health Services; Child, Preschool; China; Ciprofloxacin; Contact Tracing; Diarrhea, Infantile; Disk Diffusion Antimicrobial Tests; Drug Resistance, Multiple, Bacterial; Female; Food Microbiology; Humans; Infant; Male; Salmonella enteritidis; Salmonella Infections; Salmonella typhimurium

Administration of ceftriaxone for 5 days has been shown to be highly efficient in the treatment of severe shigellosis in children. Our study, involving 40 children, demonstrated that a 2-day course of ceftriaxone was as beneficial as a 5-day course and constituted effective therapy for children with severe shigellosis.

Topics: Adolescent; Ceftriaxone; Child; Child, Preschool; Diarrhea, Infantile; Dose-Response Relationship, Drug; Dysentery, Bacillary; Humans; Infant; Shigella flexneri; Shigella sonnei; Treatment Outcome

Topics: Ceftriaxone; Cross Infection; Diarrhea, Infantile; Humans; Infant, Newborn; Infant, Premature, Diseases; Infusions, Intravenous; Salmonella enteritidis; Salmonella Infections