rm-493 and Prader-Willi-Syndrome

α- and β-melanocyte-stimulating hormones (MSH) are derived from pro-opiomelanocortin (POMC) and are the natural agonist ligands of the melanocortin 4 receptor, a key regulator of energy homeostasis. Recent rodent and human data have implicated the MAGEL2 gene, which may regulate activation of POMC neurons, as a significant contributor to the metabolic symptoms observed in Prader-Willi Syndrome (PWS). Firstly, patients with protein truncating mutations in MAGEL2 exhibit numerous clinical characteristics of PWS. Secondly, Magel2-null mice may not normally activate MC4 receptors, as they are defective in the activation of their POMC neurons and hence may fail to normally release the POMC-derived MC4 receptor agonist ligands α- and β-MSH. Magel2-null mice represent a tractable animal model for the metabolic and appetitive imbalance seen in patients with PWS.. We tested a dose titration of the MC4 receptor agonist setmelanotide, in development for rare monogenic forms of obesity, in Magel2-null mice.. We show that Magel2-null mice are hypersensitive to the appetite suppressing and metabolic effects of setmelanotide.. Setmelanotide may be a useful investigational hormone/neuropeptide replacement therapy for PWS and rare monogenic forms of obesity exhibiting impaired function of POMC neurons.

Topics: alpha-MSH; Animals; Antigens, Neoplasm; Appetite Regulation; Dose-Response Relationship, Drug; Female; Injections, Intraperitoneal; Male; Metabolism; Mice; Mice, Inbred C57BL; Mice, Nude; Neurons; Prader-Willi Syndrome; Pro-Opiomelanocortin; Proteins; Receptor, Melanocortin, Type 4; Structure-Activity Relationship