ritonavir and Herpes-Simplex

The current outbreak of COVID-19 has spread rapidly all over the world. Respiratory droplets and contaction with infected patients are the two major transmission routes. However, the value of tear virus nucleic acid is still not clear. We dynamic detected the SARS-CoV-2 in eye sample of one COVID-19 patient with obstruction of common lacrimal ducts.. Besides the routine examination, nasopharyngeal and eye swab were continuously measured by polymerase chain reaction assay and next-generation sequencing (NGS). Gene detection was performed for drug use guidance, and flow cytometry was performed to analyse the lymphocyte subsets.. Nasopharyngeal swabs were positive for 22 days, but eye swabs were still continuously positive for 2 weeks after nasopharyngeal swabs turned negative. The low level of lymphocyte and the high level IL-6 lasted for almost 4 weeks, then became near normal. Next-generation sequencing (NGS) confirmed the existing of SARS-CoV-2, HSV1 and HHV6B virus nucleic acid. The gene detection for drug use guidance showed the genetic locus ABCB1 (3435T>C) rs1045642 belonged to type CC and it mean the efficiency of lopinavir-ritonavir would be significantly decreased. The flow cytometry of lymphocyte subsets showed PD-1. As obstruction of common lacrimal duct, positively detection in one eye for 2 weeks more after nasopharyngeal swab became negative. More eye swabs should be collected from COVID-19 patients, especially from those immunocompromised, those with eye symptoms and those had a history of ocular diseases.

Topics: Aged; Anti-Bacterial Agents; Conjunctiva; Conjunctivitis, Viral; COVID-19; COVID-19 Drug Treatment; Drug Therapy, Combination; Eye Infections, Viral; Flow Cytometry; Herpes Simplex; Herpesvirus 1, Human; Herpesvirus 6, Human; High-Throughput Nucleotide Sequencing; HIV Protease Inhibitors; Humans; Lacrimal Duct Obstruction; Lopinavir; Male; Medicine, Chinese Traditional; Moxifloxacin; Nasopharynx; Nucleic Acids; Polymerase Chain Reaction; Ritonavir; RNA, Viral; Roseolovirus Infections; SARS-CoV-2; Tears