rifampin and Leukemia--Lymphoid

Topics: Aged; Dapsone; Diagnosis, Differential; Endemic Diseases; Exanthema; Humans; Leprosy, Tuberculoid; Leukemia, Lymphoid; Male; Rifampin; Texas

A new species of Legionella that caused pneumonia in a patient with chronic lymphocytic leukemia has been characterized. The Legionella wadsworthii species nova is proposed for this bacterium. The type strain is Wadsworth 81-716A (American Type Culture Collection 33877). The new species differs phenotypically from L. pneumophila in that the predominant cellular fatty acid is methyl-12-methyltetradecanoic acid (a-15:0) rather than methyl-14-methylpentadecanoic acid (i-16:0), and in its failure to react with L. pneumophila antiserum. The clinical illness caused by L. wadsworthii was not apparently different from that seen with other legionella infections, except for prolonged fever and slow resolution of pulmonary infiltrates.

Topics: Aged; Bacteriological Techniques; Erythromycin; Humans; Legionella; Legionnaires' Disease; Leukemia, Lymphoid; Male; Pneumonia; Rifampin; Serologic Tests

Physico-chemical properties and RNA synthesis in the rat liver and human lymphocytes have been compared in a nuclear system in vitro. Human lymphocytes were isolated from blood of healthy donors and of chronic lymphocytic leukaemia patients. The isolated nuclei served as the source of polymerase and template DNA. 3H-CTP was incorporated into the acid insoluble fraction linearly for 60 min. The nuclei of lymphocytes contained small amounts of RNA and protein, and the isolation procedure was complicated. Rat liver nuclei seem to be less prone to clumping at high pH values and may incorporate much more 3H-CTP. The nuclear synthesis was compared with incorporation of 3H-rU and 32P-orthophosphate into nuclear RNA of intact lymphocytes. Normal cells easily incorporated 32-P, and in contrast leukaemic cells incorporated 3H-rU to a greater extent.

Topics: Animals; Cell Nucleus; Cell-Free System; Dactinomycin; Leukemia, Lymphoid; Liver; Lymphocytes; Rats; Rifampin; RNA; RNA, Neoplasm

Streptovaricins (Sv), ansa macrolide antibiotics, inhibited Rauscher leukemia virus (RLV) splenomegaly by 25-50%. All streptovaricins tested were effective when administered orally either by diet ad lib or by intubation from infection to time of killing. When delivered by intubation, Sv was measurable in plasma for up to 6 h. SvC, at 300 mg/kg/day, reduced mean spleen weight of infected mice from 478 plus or minus 51 (SE) mg to 300 plus or minus 55 (SE) mg. Rifampicin, at 250 mg/kg/day, had no similar activity. Decrease in caloric intake and in body-weight gain also resulted in an inhibition of RLV splenomegaly; although Sv-treated mice gained weight, the increase was usually slightly less than controls. However, mice treated with a Sv diet for a week prior to infection, after an initial period of weight loss, gained at a rate equivalent to control group, and when killed had a marked reduction in splenomegaly. The selectivity of streptovaricins and specificity for viral events was suggested by several observations: (1) Splenomegaly and mortality, induced by L1210 or a non-infective transplantable tumor of RLV origin, was not inhibited. (2) No inhibition of normal hematopoietic spleen colonies was observed. (3) Host immune responses, including cellular and humoral immunity and interferon production and action, were not inhibited. Thus, although the effect of slightly decreased weight and intake could not be unequivocally established, the findings were most compatible with a selective inhibition of RLV splenomegaly by Sv.

Topics: Animals; Antibodies, Neoplasm; Body Weight; Cell Line; Erythrocytes; Female; Graft vs Host Reaction; Hemagglutination Tests; Immunity, Cellular; Leukemia L1210; Leukemia, Experimental; Leukemia, Lymphoid; Male; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Mice, Inbred Strains; Neoplasm Transplantation; Rauscher Virus; Rifampin; Serum Albumin, Bovine; Sheep; Splenomegaly; Streptovaricin

Topics: Animals; Chloramphenicol; Drug Therapy, Combination; Drug Tolerance; Leukemia, Experimental; Leukemia, Lymphoid; Male; Mice; Mice, Inbred CBA; Mitochondria, Liver; Rauscher Virus; Rifampin; RNA, Viral

Topics: Ammonium Sulfate; Antimetabolites; Basidiomycota; Chromatin; Chromatography, Ion Exchange; DNA; DNA-Directed RNA Polymerases; Humans; Kinetics; Leukemia, Lymphoid; Lymphocytes; Magnesium; Manganese; Mycotoxins; Oligopeptides; Osmolar Concentration; Peptides, Cyclic; Rifampin; Rifamycins; Solubility; Tritium; Ultrasonics; Uracil Nucleotides

Topics: Acute Disease; Adolescent; Female; Gangrene; Humans; Leukemia, Lymphoid; Mercaptopurine; Methotrexate; Prednisolone; Pyoderma; Rifampin

Topics: Antibiotics, Antineoplastic; DNA Nucleotidyltransferases; Humans; Imines; Leukemia; Leukemia, Lymphoid; Lymphocytes; Piperazines; Rauscher Virus; Reverse Transcriptase Inhibitors; Rifampin; Structure-Activity Relationship

Topics: Animals; Avian Leukosis Virus; DNA Nucleotidyltransferases; Escherichia coli; Haplorhini; Humans; Leukemia, Lymphoid; Leukocytes; Oncogenic Viruses; Oximes; Polynucleotides; Rauscher Virus; Rifampin; RNA Viruses; RNA, Viral; Templates, Genetic

Topics: Base Sequence; Carbon Isotopes; Chromatin; DNA, Neoplasm; Escherichia coli; Humans; Kinetics; Leukemia, Lymphoid; Leukemia, Myeloid, Acute; Leukocytes; Nucleic Acid Denaturation; Nucleic Acid Hybridization; Peptide Chain Initiation, Translational; Potassium Chloride; Rifampin; RNA Nucleotidyltransferases; RNA, Neoplasm; Templates, Genetic; Thymidine; Transcription, Genetic; Tritium

Topics: Female; Herpes Simplex; Hodgkin Disease; Humans; Leukemia, Lymphoid; Male; Recurrence; Rifampin; Rifamycins; Time Factors