rifampin has been researched along with Dental-Plaque* in 2 studies
2 other study(ies) available for rifampin and Dental-Plaque
| Article | Year |
|---|---|
Enumeration of viable oral spirochetes from periodontal pockets.
We recently developed a successful method for quantifying oral anaerobic spirochetes in pure culture by a viable count. New oral spirochete medium was used with low temperature-gelling agarose in polystyrene tissue-culture flasks. We have extended the use of this method to determine the viable count of spirochetes from periodontal pockets. Sixteen subgingival plaque samples were obtained by insertion of sterile paper points into deep periodontal pockets. The points were placed into reduced transport medium at chairside, vortexed in the microbiology laboratory and aliquots of the medium inoculated into molten new oral spirochete-agarose medium (37 degrees C) containing rifampin (20 micrograms/ml) in a flask. Subsequent dilutions were made from this initial flask to other flasks containing selective medium in sequence. All flasks were incubated anaerobically. Most other subgingival bacteria were selectively inhibited by rifampin. Spirochete colonies were typically spherical and were either dense or cottony. Their identities were checked by darkfield examination. Counts of colony-forming units of cultivable spirochetes ranged from 12.5% to 28.2% of the total cultivable anaerobic flora by the method described. Topics: Colony Count, Microbial; Culture Media; Dental Plaque; Humans; Microbial Sensitivity Tests; Periodontal Pocket; Polymyxins; Rifampin; Spectinomycin; Treponema; Vancomycin | 1994 |
Alterations due to ampicillin and rifampicin in S. sanguis and S. aureus isolated from dental plaque. An electron microscopic study.
In this work we analysed the ultrastructural changes in S. aureus and S. sanguis after exposure to rifampicin and ampicillin respectively. S. aureus grown in the presence of rifampicin showed ultrastructural changes that can be summarized as follows: the peripheral cell-wall increased to three to five times the normal thickness; the cross-walls were considerably (two to ten times) thicker than normal; after 8 h of exposure a complete lysis occurred. S. sanguis grown in the presence of ampicillin showed ultrastructural changes that can be summarized as follows: aggregation and condensation of ribosomes; alterations of division with the presence of asynchronous septa; in some bacterial cells a prominent bulge was observed at one pole of the cell; after 8 h of exposure a complete lysis occurred. Topics: Ampicillin; Dental Plaque; Humans; Microbial Sensitivity Tests; Microscopy, Electron; Rifampin; Staphylococcus aureus; Streptococcus sanguis | 1986 |